Overview

  • Product name

  • Description

    Rabbit polyclonal to HUS1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Cow, Pig
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 - 243 of Human HUS1 (AAH07013).

  • Positive control

    • 293T cell lysate, CA922 xenograft

Properties

Applications

Our Abpromise guarantee covers the use of ab96297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 32 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function

    Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the HUS1 family.
  • Cellular localization

    Nucleus. Cytoplasm. In discrete nuclear foci upon DNA damage. According to PubMed:14500360, localized also in the cytoplasm. DNA damage induces its nuclear translocation. Shuttles between the nucleus and the cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Checkpoint protein HUS1 antibody
    • hHUS1 antibody
    • HUS1 (S. pombe) checkpoint homolog antibody
    • Hus1 antibody
    • HUS1 checkpoint homolog (S. pombe) antibody
    • HUS1 Checkpoint Protein antibody
    • HUS1 protein antibody
    • HUS1+ - like protein antibody
    • HUS1_HUMAN antibody
    • Hydroxyurea-sensitive 1, S. pombe, homolog of antibody
    see all

Images

  • Anti-HUS1 antibody (ab96297) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 32 kDa



    12% SDS PAGE
  • ab96297 at 1/500 dilution, staining HUS1 in paraffin-embedded CA922 xenograft.

References

This product has been referenced in:

  • Pillay N  et al. DNA Replication Vulnerabilities Render Ovarian Cancer Cells Sensitive to Poly(ADP-Ribose) Glycohydrolase Inhibitors. Cancer Cell 35:519-533.e8 (2019). Read more (PubMed: 30889383) »
  • Osorio-Zambrano WF & Davey S Chk1 Activation Protects Rad9A from Degradation as Part of a Positive Feedback Loop during Checkpoint Signalling. PLoS One 10:e0144434 (2015). Read more (PubMed: 26658951) »
See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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