Overview

  • Product name

  • Description

    Goat polyclonal to HUWE1/Mule
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Bat
  • Immunogen

    Synthetic peptide corresponding to Human HUWE1/Mule aa 2250-2300.
    Database link: Q7Z6Z7

  • Positive control

    • 293T and Hela whole cell lysates
  • General notes

     This product was previously labelled as HUWE1

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH: 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab140528 was affinity purified using an epitope specific to HUWE1/Mule immobilized on a solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab140528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500. Predicted molecular weight: 481 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1. Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair. Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene. Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN. May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation.
    • Tissue specificity

      Weakly expressed in heart, brain and placenta but not in other tissues. Expressed in a number of cell lines, predominantly in those from colorectal carcinomas.
    • Pathway

      Protein modification; protein ubiquitination.
    • Involvement in disease

      Defects in HUWE1 are the cause of mental retardation syndromic X-linked Turner type (MRXST) [MIM:300706]; also known as mental retardation and macrocephaly syndrome. MRXST shows clinical variability. Associated phenotypes include macrocephaly and variable contractures.
      A chromosomal microduplication involving HUWE1 and HSD17B10 is the cause of mental retardation X-linked type 17 (MRX17) [MIM:300705]; also known as mental retardation X-linked type 31 (MRX31). Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific X-linked mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, intellectual deficiency is the only primary symptom of non-syndromic X-linked mental retardation.
    • Sequence similarities

      Belongs to the TOM1/PTR1 family.
      Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
      Contains 1 UBA domain.
      Contains 1 UIM (ubiquitin-interacting motif) repeat.
      Contains 1 WWE domain.
    • Domain

      The HECT domain mediates inhibition of the transcriptional activity of p53.
    • Post-translational
      modifications

      Phosphorylated on tyrosine; phosphorylation is probably required for its ability to inhibit TP53 transactivation.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Cytoplasm. Nucleus. Mainly expressed in the cytoplasm of most tissues, except in the nucleus of spermatogonia, primary spermatocytes and neuronal cells (By similarity). Predominantly cytosolic or perinuclear in some colorectal carcinoma cells.
    • Information by UniProt
    • Database links

    • Alternative names

      • ARF binding protein 1 antibody
      • ARF BP1 antibody
      • ARF-binding protein 1 antibody
      • ARF-BP1 antibody
      • ARFBP1 antibody
      • BJ-HCC-24 tumor antigen antibody
      • E3 ubiquitin protein ligase HUWE1 antibody
      • E3 ubiquitin-protein ligase HUWE1 antibody
      • HECT antibody
      • HECT domain protein LASU1 antibody
      • HECT UBA and WWE domain containing protein 1 antibody
      • HectH9 antibody
      • Homologous to E6AP carboxyl terminus homologous protein 9 antibody
      • Huwe1 antibody
      • HUWE1_HUMAN antibody
      • Ib772 antibody
      • Large structure of UREB1 antibody
      • LASU1 antibody
      • Mcl 1 ubiquitin ligase E3 antibody
      • Mcl-1 ubiquitin ligase E3 antibody
      • Mule antibody
      • UBA and WWE domain-containing protein 1 antibody
      • Upstream regulatory element-binding protein 1 antibody
      • URE-B1 antibody
      • URE-binding protein 1 antibody
      • UREB1 antibody
      see all

    Images

    • All lanes : Anti-HUWE1/Mule antibody (ab140528) at 1 µg/ml

      Lane 1 : 293T whole cell lysate at 50 µg
      Lane 2 : 293T whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 481 kDa


      Exposure time: 3 minutes

    References

    ab140528 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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