Key features and details
- Rabbit polyclonal to HV1
- Suitable for: WB, ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HV1 antibody
DescriptionRabbit polyclonal to HV1
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human HV1 (C terminal) conjugated to keyhole limpet haemocyanin. (NP_115745.2).
(Peptide available as
- Human spleen and thymus tissues.
Protein previously labeled as HVCN1.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab117520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 32 kDa.Can be blocked with HV1 peptide (ab214795).|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 5 µg/ml.|
FunctionMediates the voltage-dependent proton permeability of excitable membranes. Forms a proton-selective channel through which protons may pass in accordance with their electrochemical gradient. Proton efflux, accompanied by membrane depolarization, facilitates acute production of reactive oxygen species in phagocytosis.
Tissue specificityEnriched in immune tissues, such as lymph nodes, B-lymphocytes, monocytes and spleen.
Sequence similaritiesBelongs to the hydrogen channel family.
DomainThe segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. Unlike other voltage-gated ion channels it lacks the pore domain.
The C-terminal coiled coil region mediates homodimerization and cooperative channel gating. It is essential for normal subcellular localization.
modificationsPhosphorylation may enhance channel gating.
Cellular localizationMembrane. Cell membrane. Detected mainly at intracellular membranes upon overexpression in HeLa cells (PuMed:20147290), but not in other cell types.
- Information by UniProt
- HV 1 antibody
- HV1 antibody
- HVCN 1 antibody
ab117520 has been referenced in 2 publications.
- Patel B et al. Voltage gated proton channels modulate mitochondrial reactive oxygen species production by complex I in renal medullary thick ascending limb. Redox Biol N/A:101191 (2019). PubMed: 31060879
- O'Connor PM et al. Proton channels and renal hypertensive injury: a key piece of the Dahl salt-sensitive rat puzzle? Am J Physiol Regul Integr Comp Physiol 310:R679-90 (2016). PubMed: 26843580