Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5542] to HYAL1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Product nameAnti-HYAL1 antibody [EPR5542] - BSA and Azide free
See all HYAL1 primary antibodies
DescriptionRabbit monoclonal [EPR5542] to HYAL1 - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human HYAL1 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q12794
Ab250152 is the carrier-free version of ab180143. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab250152 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab250152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa).|
FunctionMay have a role in promoting tumor progression. May block the TGFB1-enhanced cell growth.
Tissue specificityHighly expressed in the liver, kidney and heart. Weakly expressed in lung, placenta and skeletal muscle. No expression detected in adult brain. Isoform 1 is expressed only in bladder and prostate cancer cells, G2/G3 bladder tumor tissues and lymph node specimens showing tumor invasive tumors cells. Isoform 3, isoform 4, isoform 5 and isoform 6 are expressed in normal bladder and bladder tumor tissues.
Involvement in diseaseDefects in HYAL1 are the cause of mucopolysaccharidosis type 9 (MPS9) [MIM:601492]; also called hyaluronidase deficiency. MPS9 is a lysosomal storage disease characterized by high hyaluronan (HA) concentration in the serum. The clinical features are periarticular soft tissue masses, mild short stature and acetabular erosions, and absence of neurological or visceral involvement.
Sequence similaritiesBelongs to the glycosyl hydrolase 56 family.
Contains 1 EGF-like domain.
Cellular localizationSecreted. Lysosome.
- Information by UniProt
- HYAL 1 antibody
- Hyal-1 antibody
- HYAL1 antibody
All lanes : Anti-HYAL1 antibody [EPR5542] (ab180143) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : LnCap cell lysate
Lane 3 : HT-1376 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
This data was developed using ab180143, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab250152 has not yet been referenced specifically in any publications.