Anti-HYAL2 antibody (ab68608)
Key features and details
- Rabbit polyclonal to HYAL2
- Suitable for: IHC-P, IHC-Fr, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-HYAL2 antibody
See all HYAL2 primary antibodies -
Description
Rabbit polyclonal to HYAL2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IHC-Fr, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Dog, Pig, Chimpanzee -
Immunogen
Synthetic peptide corresponding to Human HYAL2 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab68607) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68608 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration. PubMed: 23560496
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IHC-Fr |
Use at an assay dependent concentration. PubMed: 23365098
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
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ICC/IF |
Use a concentration of 5 µg/ml.
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Notes |
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IHC-P
Use at an assay dependent concentration. PubMed: 23560496 |
IHC-Fr
Use at an assay dependent concentration. PubMed: 23365098 |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa). |
ICC/IF
Use a concentration of 5 µg/ml. |
Target
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Function
Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R. -
Tissue specificity
Widely expressed. No expression detected in adult brain. -
Sequence similarities
Belongs to the glycosyl hydrolase 56 family.
Contains 1 EGF-like domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 8692 Human
- Entrez Gene: 15587 Mouse
- Entrez Gene: 404697 Pig
- Entrez Gene: 64468 Rat
- Omim: 603551 Human
- SwissProt: Q12891 Human
- SwissProt: O35632 Mouse
- SwissProt: Q9Z2Q3 Rat
see all -
Alternative names
- Hyal 2 antibody
- Hyal-2 antibody
- Hyal2 antibody
see all
Images
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All lanes : Anti-HYAL2 antibody (ab68608) at 1 µg/ml
Lane 1 : Liver (Rat) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lane 4 : Human thymus tissue lysate - total protein (ab30146)
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 7 :Raji whole cell lysate (ab30124)
Lane 8 :SW480 whole cell lysate (ab3957)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa, 54 kDa (possible isoform), 58 kDa. We are unsure as to the identity of these extra bands. -
ab68608 staining HYAL2 in JEG3 cells. The cells were fixed with 100% methanol (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab68608 at 1ug/ml and ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab68608 has been referenced in 11 publications.
- Katarzyna Greda A & Nowicka D Hyaluronidase inhibition accelerates functional recovery from stroke in the mouse brain. J Neurochem 157:781-801 (2021). PubMed: 33345310
- Duan C et al. Restricted Water Intake Adversely Affects Rat Vocal Fold Biology. Laryngoscope N/A:N/A (2020). PubMed: 32658340
- Knudtson JF et al. The Hyaluronic Acid System is Intact in Menstrual Endometrial Cells in Women With and Without Endometriosis. Reprod Sci 26:109-113 (2019). PubMed: 29621955
- Yang H et al. Berberine inhibits low shear stress-induced glycocalyx degradation via modulating AMPK and p47phox/Hyal2 signal pathway. Eur J Pharmacol 856:172413 (2019). PubMed: 31152700
- Yang H et al. Hyaluronidase2 (Hyal2) modulates low shear stress-induced glycocalyx impairment via the LKB1/AMPK/NADPH oxidase-dependent pathway. J Cell Physiol 233:9701-9715 (2018). PubMed: 30078213