Anti-Hyaluronan synthase 2 antibody [4E7] (ab140671)
Key features and details
- Mouse monoclonal [4E7] to Hyaluronan synthase 2
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-Hyaluronan synthase 2 antibody [4E7]
See all Hyaluronan synthase 2 primary antibodies -
Description
Mouse monoclonal [4E7] to Hyaluronan synthase 2 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Purified recombinant fragment, corresponding to amino acids 67-170 of Human Hyaluronan synthase 2, expressed in E. coli.
-
Positive control
- Recombinant Human Hyaluronan synthase 2 protein; NTERA2 and HEK293 cell lysates; HeLa cells. Human ovarian cancer tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Contains 0.5% protein stabilizer. -
Concentration information loading...
-
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
4E7 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
1/500 - 1/2000. Predicted molecular weight: 64 kDa.
|
IHC-P | (2) |
1/200 - 1/1000.
|
ICC/IF | (2) |
1/100 - 1/500.
|
Flow Cyt |
Use 0.1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
---|
WB
1/500 - 1/2000. Predicted molecular weight: 64 kDa. |
IHC-P
1/200 - 1/1000. |
ICC/IF
1/100 - 1/500. |
Flow Cyt
Use 0.1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
-
Function
Plays a role in hyaluronan/hyaluronic acid (HA) synthesis. -
Tissue specificity
Expressed in fibroblasts. -
Pathway
Glycan biosynthesis; hyaluronan biosynthesis. -
Involvement in disease
Note=A chromosomal aberration involving HAS2 may be a cause of lipoblastomas, which are benign tumors resulting from transformation of adipocytes, usually diagnosed in children. 8q12.1 to 8q24.1 intrachromosomal rearrangement with PLAG1. -
Sequence similarities
Belongs to the nodC/HAS family. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 3037 Human
- Omim: 601636 Human
- SwissProt: Q92819 Human
- Unigene: 159226 Human
-
Alternative names
- HA synthase 2 antibody
- has2 antibody
- HAS2_HUMAN antibody
see all
Images
-
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling Hyaluronan synthase 2 with ab140671 at 1/200 dilution followed by DAB staining.
-
Immunofluorescenct analysis of HeLa cells labelling Hyaluronan synthase 2 with ab140671 at 1/100 dilution (green); Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
-
Anti-Hyaluronan synthase 2 antibody [4E7] (ab140671) at 1/500 dilution + Recombinant Human Hyaluronan synthase 2 protein
Predicted band size: 64 kDa
Expected MW is 37.5 kDa -
All lanes : Anti-Hyaluronan synthase 2 antibody [4E7] (ab140671) at 1/500 dilution
Lane 1 : NTERA2 cell lysate
Lane 2 : HEK293 cell lysate
Predicted band size: 64 kDa -
Overlay histogram showing HeLa cells stained with ab140671 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab140671, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (9)
ab140671 has been referenced in 9 publications.
- Williams R et al. Age-Related Changes in Female Scalp Dermal Sheath and Dermal Fibroblasts: How the Hair Follicle Environment Impacts Hair Aging. J Invest Dermatol 141:1041-1051 (2021). PubMed: 33326808
- Zhan H et al. Epidermal growth factor promotes stromal cells migration and invasion via up-regulation of hyaluronate synthase 2 and hyaluronan in endometriosis. Fertil Steril 114:888-898 (2020). PubMed: 32762950
- Würtemberger J et al. Growth inhibition associated with disruption of the actin cytoskeleton by Latrunculin A in rhabdomyosarcoma cells. PLoS One 15:e0238572 (2020). PubMed: 32898143
- Goody D et al. Drug Repositioning Inferred from E2F1-Coregulator Interactions Studies for the Prevention and Treatment of Metastatic Cancers. Theranostics 9:1490-1509 (2019). PubMed: 30867845
- Kim YH et al. Hyaluronic acid synthase 2 promotes malignant phenotypes of colorectal cancer cells through transforming growth factor beta signaling. Cancer Sci 110:2226-2236 (2019). PubMed: 31102316
- Meng X et al. Heterogeneous nuclear ribonucleoprotein A1 interacts with microRNA-34a to promote chondrogenic differentiation of mesenchymal stem cells. Am J Transl Res 9:1774-1782 (2017). WB ; Mouse . PubMed: 28469782
- Zhu G et al. Long noncoding RNA HAS2-AS1 mediates hypoxia-induced invasiveness of oral squamous cell carcinoma. Mol Carcinog N/A:N/A (2017). Human . PubMed: 28485478
- Chung SA et al. Pirfenidone attenuates the IL-1ß-induced hyaluronic acid increase in orbital fibroblasts from patients with thyroid-associated ophthalmopathy. Invest Ophthalmol Vis Sci 55:2276-83 (2014). Human . PubMed: 24627146
- Norsgaard H et al. Calcipotriol counteracts betamethasone-induced decrease in extracellular matrix components related to skin atrophy. Arch Dermatol Res 306:719-29 (2014). Human . PubMed: 25027750