Overview

  • Product name

    Anti-Hyaluronan synthase 2 antibody [4E7]
    See all Hyaluronan synthase 2 primary antibodies
  • Description

    Mouse monoclonal [4E7] to Hyaluronan synthase 2
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Purified recombinant fragment, corresponding to amino acids 67-170 of Human Hyaluronan synthase 2, expressed in E. coli.

  • Positive control

    • Recombinant Human Hyaluronan synthase 2 protein; NTERA2 and HEK293 cell lysates; HeLa cells. Human ovarian cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab140671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 64 kDa.
IHC-P 1/200 - 1/1000.
ICC/IF 1/100 - 1/500.
Flow Cyt Use 0.1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Plays a role in hyaluronan/hyaluronic acid (HA) synthesis.
  • Tissue specificity

    Expressed in fibroblasts.
  • Pathway

    Glycan biosynthesis; hyaluronan biosynthesis.
  • Involvement in disease

    Note=A chromosomal aberration involving HAS2 may be a cause of lipoblastomas, which are benign tumors resulting from transformation of adipocytes, usually diagnosed in children. 8q12.1 to 8q24.1 intrachromosomal rearrangement with PLAG1.
  • Sequence similarities

    Belongs to the nodC/HAS family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • HA synthase 2 antibody
    • has2 antibody
    • HAS2_HUMAN antibody
    • Hyaluronan synthase 2 antibody
    • Hyaluronate synthase 2 antibody
    • Hyaluronic acid synthase 2 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling Hyaluronan synthase 2 with ab140671 at 1/200 dilution followed by DAB staining.
  • Immunofluorescenct analysis of HeLa cells labelling Hyaluronan synthase 2 with ab140671 at 1/100 dilution (green); Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Anti-Hyaluronan synthase 2 antibody [4E7] (ab140671) at 1/500 dilution + Recombinant Human Hyaluronan synthase 2 protein

    Predicted band size: 64 kDa



    Expected MW is 37.5 kDa
  • All lanes : Anti-Hyaluronan synthase 2 antibody [4E7] (ab140671) at 1/500 dilution

    Lane 1 : NTERA2 cell lysate
    Lane 2 : HEK293 cell lysate

    Predicted band size: 64 kDa

  • Overlay histogram showing HeLa cells stained with ab140671 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab140671, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References

This product has been referenced in:

  • Goody D  et al. Drug Repositioning Inferred from E2F1-Coregulator Interactions Studies for the Prevention and Treatment of Metastatic Cancers. Theranostics 9:1490-1509 (2019). Read more (PubMed: 30867845) »
  • Kim YH  et al. Hyaluronic acid synthase 2 promotes malignant phenotypes of colorectal cancer cells through transforming growth factor beta signaling. Cancer Sci 110:2226-2236 (2019). Read more (PubMed: 31102316) »
See all 6 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Bos taurus Cell (cumulus oocyte complex)
Permeabilization
Yes - 0.25% Triton X100
Specification
cumulus oocyte complex
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 19 2017

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (liver cell type)
Specification
liver cell type
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Dec 09 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (fibroblast)
Specification
fibroblast
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 27 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Skin)
Specification
Skin
Fixative
Formalin
Antigen retrieval step
Other
Permeabilization
No
Blocking step
Goat serum as blocking agent for 40 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Apr 12 2013

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