Overview

  • Product name

    Anti-Hyaluronan synthase 2 antibody
    See all Hyaluronan synthase 2 primary antibodies
  • Description

    Rabbit polyclonal to Hyaluronan synthase 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Chinese hamster, Orangutan
  • Immunogen

    This product was produced with the following immunogens:
    corresponding to Hyaluronan synthase 2.
    (Peptide available as ab167145)

    Synthetic peptide aa 450-550.
    Database link: Q92819
    (Peptide available as ab167145)

  • Positive control

    • This antibody gave a positive signal in Human Heart tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab131364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa). Abcam recommends using milk as the blocking agent (3%).

Target

Images

  • Anti-Hyaluronan synthase 2 antibody (ab131364) at 1 µg/ml (3% milk blocking) + Human heart tissue lysate - total protein (ab29431) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 63 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 135 kDa, 35 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    Abcam recommends using milk as the blocking agent - 3%. This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab131364 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

This product has been referenced in:

  • Zhang Y  et al. MicroRNA-410 promotes chondrogenic differentiation of human bone marrow mesenchymal stem cells through down-regulating Wnt3a. Am J Transl Res 9:136-145 (2017). WB ; Human . Read more (PubMed: 28123640) »
See 1 Publication for this product

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