Key features and details
- Rabbit polyclonal to Hyaluronan synthase 2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Hyaluronan synthase 2 antibody
See all Hyaluronan synthase 2 primary antibodies
DescriptionRabbit polyclonal to Hyaluronan synthase 2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Chinese hamster, Orangutan
- This antibody gave a positive signal in Human Heart tissue lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab131364 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa). Abcam recommends using milk as the blocking agent (3%).|
FunctionPlays a role in hyaluronan/hyaluronic acid (HA) synthesis.
Tissue specificityExpressed in fibroblasts.
PathwayGlycan biosynthesis; hyaluronan biosynthesis.
Involvement in diseaseNote=A chromosomal aberration involving HAS2 may be a cause of lipoblastomas, which are benign tumors resulting from transformation of adipocytes, usually diagnosed in children. 8q12.1 to 8q24.1 intrachromosomal rearrangement with PLAG1.
Sequence similaritiesBelongs to the nodC/HAS family.
- Information by UniProt
- HA synthase 2 antibody
- has2 antibody
- HAS2_HUMAN antibody
Anti-Hyaluronan synthase 2 antibody (ab131364) at 1 µg/ml (3% milk blocking) + Human heart tissue lysate - total protein (ab29431) at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 135 kDa, 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Abcam recommends using milk as the blocking agent - 3%. This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab131364 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab131364 has been referenced in 3 publications.
- Pan Y et al. Estrogen improves the development of yak (Bos grunniens) oocytes by targeting cumulus expansion and levels of oocyte-secreted factors during in vitro maturation. PLoS One 15:e0239151 (2020). PubMed: 32941516
- Yang H et al. Berberine inhibits low shear stress-induced glycocalyx degradation via modulating AMPK and p47phox/Hyal2 signal pathway. Eur J Pharmacol 856:172413 (2019). PubMed: 31152700
- Zhang Y et al. MicroRNA-410 promotes chondrogenic differentiation of human bone marrow mesenchymal stem cells through down-regulating Wnt3a. Am J Transl Res 9:136-145 (2017). WB ; Human . PubMed: 28123640