Overview

  • Product name
    Anti-Hyaluronic acid antibody
  • Description
    Sheep polyclonal to Hyaluronic acid
  • Host species
    Sheep
  • Specificity
    ab53842 is specific for Hyaluronic acid, a mucopolysaccharide which is an integral part of the gel-like substance of animal connective tissue that serves as a lubricating and protective agent.
  • Tested applications
    Suitable for: ELISA, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Chicken, Dog, Human
    Predicted to work with: a wide range of other species
  • Immunogen

    Hyaluronic acid from human umbilical cord

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab53842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

See Abreview.

IHC-Fr Use at an assay dependent concentration.

See Abreview.

Target

  • Relevance
    Hyaluronic acid is a mucopolysaccharide which is an integral part of the gel-like substance of animal connective tissue that serves as a lubricanting and protective agent.

Images

  • ab53842 staining Hyaluronic acid in chicken cockscomb tissue sections by Immunohistochemistry (formaldehyde fixed sections). Tissue was fixed with formaldehyde and an enzymatic antigen retrieval step was performed using Pronase. Samples were then blocked with 3% H2O2 for 5 minutes at 20°C followed by incubation with the primary antibody at a 1/300 dilution for 1 hour at 20°C. ab6747 Rabbit polyclonal to Sheep IgG - H&L (HRP) was used as secondary antibody, undiluted.

    See Abreview

  • Immunohistochemical analysis of acetone-fixed 10 µm cryostat cut human eplileptic substantia alba tissue, labelling hyaluronic acid with ab53842, incubated at a dilution of 1/250 for 1 hour at 37°C in TNB diluent. Blocking was using a TNB blocking buffer for 30 minutes at 25°C. The secondary used was a polyclonal Alexa Fluor® 488 conjugate used at 1/250.

    See Abreview

References

This product has been referenced in:
  • Voutouri C & Stylianopoulos T Accumulation of mechanical forces in tumors is related to hyaluronan content and tissue stiffness. PLoS One 13:e0193801 (2018). Read more (PubMed: 29561855) »
  • Yildiz M  et al. Effects of Intracordal Estradiol and Dexamethasone Injection on Wound Healing in Vocal Fold Injuries. J Voice N/A:N/A (2018). Read more (PubMed: 29496298) »
See all 21 Publications for this product

Customer reviews and Q&As

1-10 of 23 Abreviews or Q&A

Answer

Thank you for your reply.

I am not sure what you mean by "revealed system." The secondary antibody used to generate the image on our datasheet was Rabbit polyclonal to Sheep IgG - H&L (HRP) such as ab6747, and it was used with DAB detection. Further protocol information can be found in our customer submitted Abreviews:

https://www.abcam.com/Hyaluronic-acid-antibody-ab53842-reviews.html#IHC-P

I hope this helps, please let me know if you need any other information.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (Umbilical Cord Endothelial Cells)
Permeabilization
No
Specification
Umbilical Cord Endothelial Cells
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 14 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (CNS tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid buffer
Permeabilization
Yes - Triton X
Specification
CNS tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Paraformaldehyde

Dr. Martin Broadstock

Verified customer

Submitted Feb 02 2016

Answer

We have not tested this antibody against hyaluronic acid which has been degraded by streptococcal hyaluronidase. The immunogen used was highly polymerized human hyaluronic acid as potassium salt isolated from human umbilical cord containing <2% protein. This immunogen is no longer available, so for current QC testing, the lab is using hyaluronic acid from a separate source and the antibody is still highly active, indicating protein present in the immunogen prep is not the target of this antibody. Additionally, the amount of protein in the immunizing dose would have been <50ug. During a period over 8 months in which the antibody was raised in the sheep, this amount of protein would have been unable to give the observed titer of antibody.

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Answer

Thank you for your updates.
I am very pleased to hear that finally after some optimization steps this antibody works in IHC as it is expected. Thank you for sharing your feedback with us.
If you need any further assistance in the future, please do not hesitate to contact me.
Good luck with your research!

Read More

Answer

Thank you for your response.
Normally we do not send a free vial if our customer has run out of the antibody. However, this is a special case and I understand your concern so take your request as an exemption.
This is to let you know that I have placed a new order for you - for one vial of ab53842 as a free of charge replacement and the new order number is 1147239.
I hope the second vial will allow you to finish the experiments. Please keep in touch and do let me know how you are getting on with this product.

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Answer

Thank you for your enquiry regarding ab53842 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.
I would like to reassure you that our Abpromise applies to your complaint since you purchased this product within the guarantee period. This means that in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.
This antibody has been tested in ELISA application in the Lab so we only have supporting data for ELISA. IHC-P, IHC-Fr data come from a specific publication you may wish to take a look at to get some further information:
Hoganson DM et al. The retention of extracellular matrix proteins and angiogenic and mitogenic cytokines in a decellularized porcine dermis. Biomaterials 31:6730-7 (2010). IHC-P; Pig. Read more (PubMed: 20576289) »
http://www.ncbi.nlm.nih.gov/pubmed/20576289?dopt=Abstract
I hope this will be useful for you. Should you still have any problem with this antibody after following these suggestions, then please do not hesitate to contact our Technical Department again.

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Answer

Thank you for your patience.

The immunogen used for this antibody was product H1504 from Sigma Aldrich. The antibody binds simply because hyaluronic acid is antigenic; it is the same principle for how antibodies naturally bind bacteria (ie they bind to the LPS rather than to an actual protein). Unfortunately the epitope of this polyclonal antibody is not determined so we will not be able to provide this info.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

I am sorry to hear that you have been experiencing problems using this product in the application that you wish.
I would like to reassure you that our Abpromise applies to your complaint since you purchased this product within the guarantee period. This means that in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.
In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application used. Often it is possible to make suggestions that may help resolve problems experienced using a particular product.
All our customer feedback, including complaints are monitored weekly by our in house technical support team. If a product is at fault the technical support team will consider removing the product from our catalogue in order to avoid future customer inconvenience.
Please could you provide some further details of the protocol used and complete the following form (attached as a word document). It would be much appreciated if you could attach an image to the response.
Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.

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Answer

Thank you for contacting us yesterday and your interest in our products.

As discussed over the phone, the anti-Hyaluronic acid antibody (ab53842) has not as yet been used in ICC as you are hoping to do. This does not mean that it would not work, simply that we have not had any reports supporting it yet.

As discussed over the phone, unfortunately we are not able to provide sample sizes of any of our products. This is due to the logistics that would be involved in this, with such a large catalogue this simply isn't feasible. However, we do offer a testing discount scheme. This offer means that if you test the antibodywith ICC staining and share the results with us through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price off any product in our catalogue). More information on this offer can be found here:

https://www.abcam.com/collaboratordiscount

The only constraint to this offer is that the antibody to be tested must be purchased, tested, the Abreview submitted and the free primary antibody claimed within a 4 month period.

As discussed over the phone, I have generated the testing discount code for you which is as follows:

xxxxx


Please quote this code in the "Additional comments" section when submitting your Abreview. Once the Abreview has been submitted the code will become activated and you can quote the code when placing the order for your free product. This code expires on the 29th of October 2012.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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1-10 of 23 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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