• Product name
    Hydrogen Peroxide Assay Kit
    See all Hydrogen Peroxide kits
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type
  • Sensitivity
    > 0.04 µM
  • Assay time
    1h 00m
  • Product overview

    Hydrogen Peroxide Assay Kit (ab102500) is a highly sensitive, simple, direct and HTS-ready colorimetric and fluorometric assay for measuring H2O2 in biological samples. In the presence of Horse Radish Peroxidase (HRP), the OxiRed Probe reacts with H2O2 to produce product with color (λmax = 570 nm) and red-fluorescent (Ex/Em=535/587 nm). The kit can perform 200 reactions by fluorometric method or 100 reactions by colorimetric method. The detection limit can be as low as 2 pmol per assay (or 40 nM concentration) of H2O2 in the sensitive fluorometric assay.

    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Hydrogen Peroxide (H2O2) is a reactive oxygen metabolic byproduct that serves as a key regulator for a number of oxidative stress-related states. Functioning through NF-kappaB and other factors, hydroperoxide-mediated pathways have been linked to asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neuro-degenerative diseases, Down's syndrome and immune system diseases.



  • H2O2 Colorimetric Standard Curve
  • H2O2 Fluorimetric Standard Curve



This product has been referenced in:
  • Kleniewska P & Pawliczak R Influence of Synbiotics on Selected Oxidative Stress Parameters. Oxid Med Cell Longev 2017:9315375 (2017). Read more (PubMed: 28286605) »
  • Tian Z  et al. Cigarette Smoke Impairs A2A Adenosine Receptor Mediated Wound Repair through Up-regulation of Duox-1 Expression. Sci Rep 7:44405 (2017). Read more (PubMed: 28337995) »

See all 11 Publications for this product

Customer reviews and Q&As

I can confirm that the kit ab102522 and ab102500 can be used to assay xanthine oxidase and Hydrogen peroxide in cells. I would suggest starting with at least 1-2 million cells. Please follow the following protocol;

2X106 cells, suspend the ce...

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As recommended on the protocol, an amount ranging from 2 to 50ul should be added to each well, and fill it up to 50ul with assay buffer.

As the concentration of H2O2 in your samples is unknown, you may need to run a quick test before...

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The kit ab102500 is suitable with many type of biological fluids and extracts such as cell lysates and tissue culture supernatant.

Frozen sample can be used with this kit so once the extract is prepared these should be immediately frozen at -...

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Thank you for contacting us.
Since we mention only a 10 min incubation time for this reaction, I would recommend taking the reading right after. Waiting for more time might result in increase in the background signal and later drop in the total si...

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Thank you for contacting us.

Please buy the requested product ab167452.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

I have sent a request to my colleague for adding this product in catalogue. I will let you know the catalogue number soon.


Thank you for contacting us.

We can provide one bottle of assay buffer for £90. Please let us know if you are interested in buying.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any ...

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Thank you for contacting us.
This kit can actually be used both colorimetrically and fluorometrically. In general fluorometric analysis is ˜10 times more sensitive than colorimetric analysis. The detection limit with this assay can be as low as 2 ...

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We recommend the use of the 10 kDa filters irrespective of your immediate or later usage of the lysed samples. The filters help in separating out proteins which can chew up the analyte. You most likely will not get efficient results without the fliters...

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Thank you for contacting us.

You can use the H2O2 assay buffer in the kit as the lysis buffer for cells.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

1-10 of 18 Abreviews or Q&A


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