Key features and details
- Assay type: Cell-based
- Detection method: Fluorescent
- Platform: Fluorescence microscope
- Assay time: 30 min
- Sample type: Adherent cells, Suspension cells
- Sensitivity: 3 µM
Product nameHydrogen Peroxide Assay Kit (Cell-based)
See all Hydrogen Peroxide kits
Sample typeAdherent cells, Suspension cells
Assay time0h 30m
Species reactivityReacts with: Mammals, Other species
Hydrogen Peroxide Assay Kit (Cell-based) (ab138874) uses our AbGreen Indicator to quantify hydrogen peroxide in live cells. The indicator is cell-permeable, and generates green fluorescence when it reacts with hydrogen peroxide.
The assay is in a “mix and read” assay format that is compatible with HTS liquid handling instruments and a 96-well or 384-well plate format. It provides a sensitive, one-step fluorometric assay to detect as little as 0.3 nanomoles of H2O2 in a 100 µL assay volume (3 µM). Results can be read by either a fluorescence microplate reader at Ex/Em = 490/520 nm for H2O2 detection in solution or a fluorescence microscopy for live cell H2O2 imaging.
Hydrogen peroxide assay protocol summary:
- add samples and standards to wells
- add green indicator solution
- analyze with microplate reader for 15-60 min.
Alternatively, for live cell assay:
- add green indicator solution to cells
- incubate for 5-60 min whilst analyzing with microplate reader or fluorescence microscope
Hydrogen peroxide (H2O2) is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states. It is involved in many biological events that are linked to asthma, atherosclerosis, diabetic vasculopathy, osteoporosis, a number of neurodegenerative diseases and Down’s syndrome. The measurement of this reactive species is helpful for determining how oxidative stress modulates various intracellular pathways.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 200 tests AbGreen Indicator 1 vial Assay Buffer 1 x 20ml DMSO 1 x 200µl Hydrogen Peroxide Solution 1 x 200µl
RelevanceHydrogen Peroxide (H2O2) is a reactive oxygen metabolic byproduct that serves as a key regulator for a number of oxidative stress-related states. Functioning through NF-kB and other factors, hydroperoxide-mediated pathways have been linked to asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neuro-degenerative diseases, Down’s syndrome and immune system diseases.
ab138874 has been referenced in 2 publications.
- Varadaraj K & Kumari SS Lens aquaporins function as peroxiporins to facilitate membrane transport of hydrogen peroxide. Biochem Biophys Res Commun 524:1025-1029 (2020). PubMed: 32063362
- Zhou T et al. Growth suppression and apoptosis-like cell death in Microcystis aeruginosa by H2O2: A new insight into extracellular and intracellular damage pathways. Chemosphere 211:1098-1108 (2018). PubMed: 30223325