Hydrogen Peroxide Blocking Reagent (ab64218)

Reviews (3)Q&A (1)References (3)

Overview

  • Product name
    Hydrogen Peroxide Blocking Reagent
  • Tested applications
    Suitable for: IHC-P, IHC-Frmore details
  • General notes

    Incubate tissue section for 5-10 minutes at room temperature prior to application of the primary antibody. After incubation rinse twice in buffer.

    Note: The concentration of hydrogen peroxide in this product is 0.3%.

    Find more IHC kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab64218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.

Images

  • Immunohistochemistry (Frozen sections) - Hydrogen Peroxide Blocking Reagent (ab64218)
    Immunohistochemistry (Frozen sections) - Hydrogen Peroxide Blocking Reagent (ab64218)Selby M.J et al., PLoS One 11(9), Fig 2. doi: 10.1371/journal.pone.0161779.

    Immunohistochemical (frozen) analysis of MC38 tumor sections labeling CD3 with ab16669 at 1/400 dilution. Sections were fixed with acetone, treated with peroxidase block (ab64218) to quench endogenous peroxidase, and then further blocked with a 10% goat serum and 5% BSA solution. CD3 positive T cells were detected using ab80437. Invasive margin (top) shows sections derived from the periphery of the tumor and interior (bottom) shows sections from within the tumor.

     

References

This product has been referenced in:
  • Akca G  et al. The protective effect of astaxanthin against cisplatin-induced nephrotoxicity in rats. Biomed Pharmacother 100:575-582 (2018). Read more (PubMed: 29494988) »
  • Farrow N  et al. Epithelial disruption: a new paradigm enabling human airway stem cell transplantation. Stem Cell Res Ther 9:153 (2018). Read more (PubMed: 29895311) »
See all 3 Publications for this product

Publishing research using ab64218? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews

really good working kit

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
We used this kit to reduce background staining produced by endogenous peroxidase .
Its works good and is easy to use, because of the dropper bottle.
We used the kit as a ready to use-kit and incubated the samples for 10minutes with the hydrogen peroxidase blocking reagent.

Abcam user community

Verified customer

Submitted Mar 12 2018

IHC-P with Ki67 antibody using H2O2 blocking reagant

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
abreview image
H2O2 blocking reagant was used prior to protein blocking, and primary antibody was diluted 1:100 and incubated at RT for 1 hr. As the image indicates, the peroxidase activity was significantly blocked and there was minimal background staining.

Abcam user community

Verified customer

Submitted Aug 16 2016

Hydrogen peroxidase Blocking Solution

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
abreview image
Using HRP conjugated antibody may result in high, non-specific background staining. The non-specific background can be significantly reduced by pre-treatment of tissues with hydrogen peroxide prior to incubation with HRP conjugated antibody. Pictures show mouse gastrocnemius staining with no pre-treatment and pre-treatment with Hydrogen Peroxide Blocking solution from abcam. Results shows a significant improvement with an abolished background staining.

Dr. Francesco Elia Marino

Verified customer

Submitted Jul 23 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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