Hydrogen Peroxide Blocking Reagent (ab64218)
- Datasheet
- References (7)
- Protocols
Overview
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Product name
Hydrogen Peroxide Blocking Reagent -
Tested applications
Suitable for: IHC-P, IHC-Frmore details -
General notes
Hydrogen Peroxide Blocking Reagent ab64218 for use in IHC with detection based on HRP / peroxidase.
Note: The concentration of hydrogen peroxide in this product is 0.3%.
IHC protocol suitable for use with Hydrogen Peroxide Blocking Reagent:
For frozen sections, skip steps 1 and 2.1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.
2. Use appropriate antigen retrieval buffer or enzyme (primary antibody dependent) to treat sections. Wash 3 times in buffer.
3. Add enough hydrogen peroxide blocking solution ab64218 to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer. If necessary, use avidin biotin blocking.
4. Apply protein block (or normal serum from same species as secondary antibody) and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash once in buffer.
5. Apply primary antibody in antibody diluent and incubate.
6. Wash 4 times in buffer. Incubate slide with biotinylated secondary antibody (or HRP polymer secondary antibody and skip step 7). Wash 4 times in buffer.
7. Apply streptavidin-HRP and incubate for 10 minutes at room temperature.
8. Rinse 4 times in buffer. Place slide in DAB substrate or AEC substrate and incubate until desired color is achieved (1-10 mins). Rinse 4 times in buffer.
9. Add enough drops of hematoxylin to cover the section. Incubate for 1 minute.
10. Rinse 7-8 times in tap water. Add mounting medium to cover the section.
Find complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
Properties
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Form
Liquid -
Storage instructions
Store at +4°C. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 0.3% Hydrogen peroxide -
Concentration information loading... -
Research areas
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Relevance
Hydrogen Peroxide Blocking Reagent is intended for use in peroxidase-based immunohistochemical staining procedures on cell preparations, frozen tissue sections, and paraffin-embedded tissue sections. -
Alternative names
- Hydrogen peroxide block
Applications
Our Abpromise guarantee covers the use of ab64218 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent dilution. | |
| IHC-Fr | Use at an assay dependent dilution. |
Images
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Immunohistochemistry (Frozen sections) - Hydrogen Peroxide Blocking Reagent (ab64218)Selby M.J et al., PLoS One 11(9), Fig 2. doi: 10.1371/journal.pone.0161779. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunohistochemical (frozen) analysis of MC38 tumor sections labeling CD3 with ab16669 at 1/400 dilution. Sections were fixed with acetone, treated with peroxidase block (ab64218) to quench endogenous peroxidase, and then further blocked with a 10% goat serum and 5% BSA solution. CD3 positive T cells were detected using ab80437. Invasive margin (top) shows sections derived from the periphery of the tumor and interior (bottom) shows sections from within the tumor.
References
This product has been referenced in:
- Akca G et al. The protective effect of astaxanthin against cisplatin-induced nephrotoxicity in rats. Biomed Pharmacother 100:575-582 (2018). Read more (PubMed: 29494988) »
- Farrow N et al. Epithelial disruption: a new paradigm enabling human airway stem cell transplantation. Stem Cell Res Ther 9:153 (2018). Read more (PubMed: 29895311) »
