Anti-HYPE antibody (ab237521)
Key features and details
- Rabbit polyclonal to HYPE
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-HYPE antibody
See all HYPE primary antibodies -
Description
Rabbit polyclonal to HYPE -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment corresponding to Human HYPE aa 50-200.
Database link: Q9BVA6 -
Positive control
- WB: A549 and HeLa whole cell lysates; Rat kidney lysate. IHC-P: Human small intestine and kidney tissue. ICC/IF: PC-3 cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
-
Purity
Protein G purified -
Purification notes
Purity greater than 95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab237521 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/5000. Predicted molecular weight: 52 kDa.
|
|
IHC-P |
1/500 - 1/1000.
|
|
ICC/IF |
1/50 - 1/200.
|
Notes |
---|
WB
1/500 - 1/5000. Predicted molecular weight: 52 kDa. |
IHC-P
1/500 - 1/1000. |
ICC/IF
1/50 - 1/200. |
Target
-
Function
Adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins. Able to inactivate Rho GTPases in vitro by adding AMP to RhoA, Rac and Cdc42. It is however unclear whether it inactivates GTPases in vivo and physiological substrates probably remain to be identified. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the fic family.
Contains 1 fido domain.
Contains 2 TPR repeats. -
Domain
The fido domain mediates the adenylyltransferase activity. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 11153 Human
- Entrez Gene: 288741 Rat
- SwissProt: Q9BVA6 Human
- SwissProt: Q6AY47 Rat
- Unigene: 661891 Human
- Unigene: 162153 Rat
-
Alternative names
- Adenosine monophosphate-protein transferase FICD antibody
- AMPylator FICD antibody
- FIC domain containing antibody
see all
Images
-
All lanes : Anti-HYPE antibody (ab237521) at 1/500 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Rat kidney tissue lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 52 kDa -
Paraffin-embedded human small intestine tissue stained for HYPE using ab237521 at 1/500 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
-
Paraffin-embedded human kidney tissue stained for HYPE using ab237521 at 1/500 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
-
PC-3 (Human prostate adenocarcinoma cell line) cells stained for HYPE (green) using ab237521 at 1/166 dilution in ICC/IF. Secondary antibody is an Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L). The nuclear counter stain is DAPI (blue).
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab237521 has not yet been referenced specifically in any publications.