Anti-HYPE antibody (ab67163)
Key features and details
- Mouse polyclonal to HYPE
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HYPE antibody
See all HYPE primary antibodies -
Description
Mouse polyclonal to HYPE -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human HYPE. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- Transfected 293T cell lysate, human placenta tissue lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab67163 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
Target
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Function
Adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins. Able to inactivate Rho GTPases in vitro by adding AMP to RhoA, Rac and Cdc42. It is however unclear whether it inactivates GTPases in vivo and physiological substrates probably remain to be identified. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the fic family.
Contains 1 fido domain.
Contains 2 TPR repeats. -
Domain
The fido domain mediates the adenylyltransferase activity. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 11153 Human
- SwissProt: Q9BVA6 Human
- Unigene: 661891 Human
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Alternative names
- Adenosine monophosphate-protein transferase FICD antibody
- AMPylator FICD antibody
- FIC domain containing antibody
see all
Images
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Anti-HYPE antibody (ab67163) at 1/500 dilution + human placenta tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa -
All lanes : Anti-HYPE antibody (ab67163) at 1/500 dilution
Lane 1 : Transfected 293T cell lysate
Lane 2 : non Transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
Datasheets and documents
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Datasheet download
References (1)
ab67163 has been referenced in 1 publication.
- Sanyal A et al. Alpha-Synuclein Is a Target of Fic-Mediated Adenylylation/AMPylation: Possible Implications for Parkinson's Disease. J Mol Biol 431:2266-2282 (2019). PubMed: 31034889