Overview

  • Product name

    Anti-HYPK antibody
  • Description

    Rabbit polyclonal to HYPK
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human HYPK aa 25-75. The exact sequence is proprietary. Isoform 2.
    Database link: Q9NX55

  • Positive control

    • IP: HeLa whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab228820 was affinity purified using an epitope specific to HYPK immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab228820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.

Use at 8 - 15 µl/mg lysate.

Target

  • Function

    Has a chaperone-like activity preventing polyglutamine (polyQ) aggregation of HTT. Protects against HTT polyQ-mediated apoptosis in Neuro2a neuronal cells. Required for optimal NAA10-NAA15 complex-mediated N-terminal acetylation.
  • Cellular localization

    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Huntingtin yeast partner K antibody
    • Huntingtin-interacting protein K antibody
    • HYPK antibody
    • HYPK_HUMAN antibody

Images

  • HYPK was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab228820 at 8 µl per reaction. Western blot was performed from the immunoprecipitate using ab228820 at a 1/400 dilution.

    Lane 1: ab228820 IP in HeLa whole cell lysate.
    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 75 seconds.

References

ab228820 has not yet been referenced specifically in any publications.

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