Key features and details
- Rabbit polyclonal to HYPK
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HYPK antibody
DescriptionRabbit polyclonal to HYPK
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human HYPK aa 25-75. The exact sequence is proprietary. Isoform 2.
Database link: Q9NX55
- IP: HeLa whole cell lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab228820 was affinity purified using an epitope specific to HYPK immobilized on solid support.
Our Abpromise guarantee covers the use of ab228820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
Use at 8 - 15 µl/mg lysate.
FunctionHas a chaperone-like activity preventing polyglutamine (polyQ) aggregation of HTT. Protects against HTT polyQ-mediated apoptosis in Neuro2a neuronal cells. Required for optimal NAA10-NAA15 complex-mediated N-terminal acetylation.
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
- Huntingtin yeast partner K antibody
- Huntingtin-interacting protein K antibody
- HYPK antibody
- HYPK_HUMAN antibody
HYPK was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab228820 at 8 µl per reaction. Western blot was performed from the immunoprecipitate using ab228820 at a 1/400 dilution.
Lane 1: ab228820 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 75 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228820 has not yet been referenced specifically in any publications.