Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Lysate, Urine
- Sensitivity: 0.001 %
Product nameHypochlorite Assay Kit (Colorimetric)
See all Hypochlorite kits
Sample typeUrine, Serum, Plasma, Other biological fluids, Cell Lysate, Tissue Lysate
Species reactivityReacts with: Other species
Hypochlorite Detection Kit (Colorimetric) (ab219929) provides a sensitive assay for measuring hypochlorite (HClO, hypochlorous acid) with high specificity in cell and tissue lysates and biological fluids. The hypochlorite sensor used in the assay selectively reacts with hypochlorite (hypochlorous) to generate a red color product which can be measured with an absorbance microplate reader at OD 555 nm.
Hypochlorite anion (ClO-) and its protonated form, hypochlorous acid (HClO) are critical reactive oxygen species (ROS) in biological systems. Uncontrolled production of hypochlorite (hypochlorous acid) can lead to tissue damage and diseases including arthritis, renal failure and cancers. In addition, sodium hypochlorite (NaClO) has been widely used as a bleaching agent for surface cleaning, odor removal and water disinfection in our daily lives. Exposure to large amount of sodium hypochlorite can lead to poisoning with the symptoms of serious breathing problems, stomach irritation, redness and pain on skin and eye. Therefore, highly selective and sensitive detection of hypochlorite (hypochlorous acid) is of toxicological and environmental importance.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 200 tests Assay Buffer 1 x 20ml DMSO 1 x 600µl Hypochlorite Sensor 1 vial Hypochlorite Standard 1 x 300µl
ab219929 has been referenced in 1 publication.
- Boudousquié C et al. Development and Optimization of a GMP-Compliant Manufacturing Process for a Personalized Tumor Lysate Dendritic Cell Vaccine. Vaccines (Basel) 8:N/A (2020). PubMed: 31947581