Overview

  • Product name

    Hypochlorite Assay Kit (Colorimetric)
    See all Hypochlorite kits
  • Detection method

    Colorimetric
  • Sample type

    Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Tissue Lysate
  • Assay type

    Quantitative
  • Sensitivity

    0.001 %
  • Species reactivity

    Reacts with: Other species
  • Product overview

    Hypochlorite Detection Kit (Colorimetric) (ab219929) provides a sensitive assay for measuring hypochlorite (HClO, hypochlorous acid) with high specificity in cell and tissue lysates and biological fluids. The hypochlorite sensor used in the assay selectively reacts with hypochlorite (hypochlorous) to generate a red color product which can be measured with an absorbance microplate reader at OD 555 nm.

  • Notes

    Hypochlorite anion (ClO-) and its protonated form, hypochlorous acid (HClO) are critical reactive oxygen species (ROS) in biological systems. Uncontrolled production of hypochlorite (hypochlorous acid) can lead to tissue damage and diseases including arthritis, renal failure and cancers. In addition, sodium hypochlorite (NaClO) has been widely used as a bleaching agent for surface cleaning, odor removal and water disinfection in our daily lives. Exposure to large amount of sodium hypochlorite can lead to poisoning with the symptoms of serious breathing problems, stomach irritation, redness and pain on skin and eye. Therefore, highly selective and sensitive detection of hypochlorite (hypochlorous acid) is of toxicological and environmental importance.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 200 tests
    Assay Buffer 1 x 20ml
    DMSO 1 x 600µl
    Hypochlorite Sensor 1 vial
    Hypochlorite Standard 1 x 300µl

Images

  • Typical Hypochlorite standard calibration curve. Hypochlorite was measured with in a 96 well clear bottom plate. As low as 0.001% (10 ppm) sodium hypochlorite (NaClO) was detected with 3-5 minutes incubation.

Protocols

References

ab219929 has not yet been referenced specifically in any publications.

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