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Other Immunogen Type corresponding to Pig Hypophosphorylated Neurofilament H (C terminal). C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.
Our Abpromise guarantee covers the use of ab82259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 112 kDa).|
|IHC-P||Use a concentration of 1 - 2 µg/ml.|
|IHC-Fr||Use a concentration of 1 - 2 µg/ml. Acetone fixed.|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration.|
ab82259 staining Hypophosphorylated Neurofilament H in Rat nerve tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde, permeablized with 0.2 Triton-X and blocked with 10% serum. The sample was incubated with primary antibody (1/50 in PBS plus 1x Casien) at 25°C for 2 hours. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal(1/200) was used as the secondary antibody. GFAP was labelled with Texas Red.
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