Question (45562) | Anti-Hypophosphorylated Neurofilament H antibody [N52] (ab82259)

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Question

Hello, I was wondering if ab82259 can be used on paraffinized tissue or if the tissue has to be deparrafinized before use.

Answer

Thank you for contacting us.

Yes, the antibody ab82259 can be used to stain sections of paraffin-embedded tissue but the paraffin will need to be removed from the sections. Without paraffin removal, aqueous solutions will not diffuse into the tissue.

This is typically accomplished by moving the slides through a series of solvents, for 3 minutes each: 2 containers of xylene (or a non-toxic alternative such as Slide Brite), and a graded series of ethanol:water mixtures (100% ethanol, 80%, 70%, 50%, distilled water).

At this point, the sections are "re-hydrated" and are ready for immunostaining.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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