Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Hypophosphorylated Neurofilament H antibody [NF-200]

Excellent
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (nerve)
Specification
nerve
Fixative
Formaldehyde
Permeabilization
Yes - 0.2% triton

Other product details

Dilution
1/50
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS plus 1xcasein

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor 488 goat anti-mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200

Additional data

Additional Notes
double immunefluorescence staining: NF200 labeled with AF488, GFAP labeled with Texas Red.

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Submitted May 08 2013

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