Immunocytochemistry/ Immunofluorescence abreview for Anti-Hypophosphorylated Neurofilament H antibody [NF-200]

Poor
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (rat motor neuron primary cells from embryo spiral)
Permeabilization
Yes - 0.2% triton
Specification
rat motor neuron primary cells from embryo spiral
Blocking step
Leica Universal IHC blocking as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
2 hour(s) and 45 minute(s) · Temperature: 25°C · Diluent: PBS plus 1x casein
Dilution
1/50

Secondary antibody

Name
Non-Abcam antibody was used: Leica poly-HRP anti-mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
No cross reaction was detected (while the cells are strongly positive to beta-tubulin III, review submitted). The results were verified by many trials, including regular immunofluorescence method with low temp (4oC) and overnight incubation.

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Submitted Aug 06 2015

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