Key features and details
- Rabbit polyclonal to Hypothetical Protein FLJ21908/RPAP3
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Hypothetical Protein FLJ21908/RPAP3 antibody
DescriptionRabbit polyclonal to Hypothetical Protein FLJ21908/RPAP3
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Hypothetical Protein FLJ21908/RPAP3 aa 400-450. The exact sequence is proprietary.
Database link: Q9H6T3
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.
This product was previously labelled as Hypothetical Protein FLJ21908
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab241410 was affinity purified using an epitope specific to Hypothetical Protein FLJ21908/RPAP3 immobilized on solid support.
Our Abpromise guarantee covers the use of ab241410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 76 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionForms an interface between the RNA polymerase II enzyme and chaperone/scaffolding protein, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation.
Sequence similaritiesBelongs to the RPAP3 family.
Contains 7 TPR repeats.
- Information by UniProt
- FLJ21908 antibody
- RNA polymerase II associated protein 3 antibody
- RNA polymerase II-associated protein 3 antibody
All lanes : Anti-Hypothetical Protein FLJ21908/RPAP3 antibody (ab241410) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 76 kDa
Exposure time: 30 seconds
Lysates prepared using NETN lysis buffer.
Hypothetical Protein FLJ21908/RPAP3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241410 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab241410 at 0.4 µg/ml.
Lane 1: ab241410 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Lysates prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241410 has not yet been referenced specifically in any publications.