Anti-Hypothetical Protein FLJ21908/RPAP3 antibody (ab241410)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Hypothetical Protein FLJ21908/RPAP3 antibody -
Description
Rabbit polyclonal to Hypothetical Protein FLJ21908/RPAP3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Hypothetical Protein FLJ21908/RPAP3 aa 400-450. The exact sequence is proprietary.
Database link: Q9H6T3 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.
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General notes
This product was previously labelled as Hypothetical Protein FLJ21908
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab241410 was affinity purified using an epitope specific to Hypothetical Protein FLJ21908/RPAP3 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab241410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 76 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Forms an interface between the RNA polymerase II enzyme and chaperone/scaffolding protein, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation. -
Sequence similarities
Belongs to the RPAP3 family.
Contains 7 TPR repeats. - Information by UniProt
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Database links
- Entrez Gene: 79657 Human
- Omim: 611477 Human
- SwissProt: Q9H6T3 Human
- Unigene: 437855 Human
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Alternative names
- FLJ21908 antibody
- RNA polymerase II associated protein 3 antibody
- RNA polymerase II-associated protein 3 antibody
see all
Images
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Western blot - Anti-Hypothetical Protein FLJ21908/RPAP3 antibody (ab241410)All lanes : Anti-Hypothetical Protein FLJ21908/RPAP3 antibody (ab241410) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 76 kDa
Exposure time: 30 secondsLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-Hypothetical Protein FLJ21908/RPAP3 antibody (ab241410)Hypothetical Protein FLJ21908/RPAP3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241410 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab241410 at 0.4 µg/ml.
Lane 1: ab241410 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Lysates prepared using NETN lysis buffer.
Datasheets and documents
References
ab241410 has not yet been referenced specifically in any publications.
