Anti-Hypothetical Protein FLJ21908/RPAP3 antibody (ab241410)
Key features and details
- Rabbit polyclonal to Hypothetical Protein FLJ21908/RPAP3
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Hypothetical Protein FLJ21908/RPAP3 antibody -
Description
Rabbit polyclonal to Hypothetical Protein FLJ21908/RPAP3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Hypothetical Protein FLJ21908/RPAP3 aa 400-450. The exact sequence is proprietary.
Database link: Q9H6T3 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.
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General notes
This product was previously labelled as Hypothetical Protein FLJ21908
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab241410 was affinity purified using an epitope specific to Hypothetical Protein FLJ21908/RPAP3 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab241410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 76 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Forms an interface between the RNA polymerase II enzyme and chaperone/scaffolding protein, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation. -
Sequence similarities
Belongs to the RPAP3 family.
Contains 7 TPR repeats. - Information by UniProt
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Database links
- Entrez Gene: 79657 Human
- Omim: 611477 Human
- SwissProt: Q9H6T3 Human
- Unigene: 437855 Human
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Alternative names
- FLJ21908 antibody
- RNA polymerase II associated protein 3 antibody
- RNA polymerase II-associated protein 3 antibody
see all
Images
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Western blot - Anti-Hypothetical Protein FLJ21908/RPAP3 antibody (ab241410)All lanes : Anti-Hypothetical Protein FLJ21908/RPAP3 antibody (ab241410) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 76 kDa
Exposure time: 30 secondsLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-Hypothetical Protein FLJ21908/RPAP3 antibody (ab241410)Hypothetical Protein FLJ21908/RPAP3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241410 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab241410 at 0.4 µg/ml.
Lane 1: ab241410 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Lysates prepared using NETN lysis buffer.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab241410 has not yet been referenced specifically in any publications.
