Overview

  • Product name

  • Description

    Goat polyclonal to I-FABP
  • Host species

    Goat
  • Tested applications

    Suitable for: ELISA, WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Cat, Human
    Predicted to work with: Rat, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to Human I-FABP aa 121-132 (C terminal).
    Sequence:

    C-EGVEAKRIFKKD


    (Peptide available as ab115238)

  • Positive control

    • Recombinant Human I-FABP protein (ab84197) can be used as a positive control in WB. Human Duodenum lysate.
  • General notes

    Previously labelled as intestinal FABP.

Properties

Applications

Our Abpromise guarantee covers the use of ab60272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution. Antibody detection limit dilution 1:64000.
WB Use a concentration of 0.001 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 15 kDa).
IHC-P Use a concentration of 2 - 3 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 23236492

Target

  • Function

    FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
  • Tissue specificity

    Expressed in the small intestine and at much lower levels in the large intestine. Highest expression levels in the jejunum.
  • Sequence similarities

    Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • Domain

    Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • FABP 2 antibody
    • FABP2 antibody
    • FABPI antibody
    • FABPI_HUMAN antibody
    • Fatty acid binding protein 2 intestinal antibody
    • Fatty acid binding protein antibody
    • Fatty acid binding protein intestinal antibody
    • Fatty acid-binding protein 2 antibody
    • Fatty acid-binding protein antibody
    • I FABP antibody
    • I-FABP antibody
    • IFABP antibody
    • intestinal antibody
    • intestinal FABP antibody
    • Intestinal fatty acid binding protein 2 antibody
    • Intestinal-type fatty acid-binding protein antibody
    • MGC133132 antibody
    • OTTHUMP00000163925 antibody
    see all

Images

  • ab60272 antibody (0.001µg/ml) staining of I-FABP in Human Duodenum lysate (35µg protein in RIPA buffer)
    Predicted band size: 15 kDa
    Observed band size: 16 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected by chemiluminescence.
  • ab60272 staining I-FABP in Human small intestine by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue sections underwent antigen retrieval by steam with citrate buffer pH 6.0. The AP staining procedure was used for detection.

References

This product has been referenced in:

  • Ziegler AL  et al. Epithelial restitution defect in neonatal jejunum is rescued by juvenile mucosal homogenate in a pig model of intestinal ischemic injury and repair. PLoS One 13:e0200674 (2018). Read more (PubMed: 30138372) »
  • Treutlein G  et al. Novel potential interacting partners of fibronectin in spontaneous animal model of interstitial cystitis. PLoS One 7:e51391 (2012). WB, ICC/IF ; Cat . Read more (PubMed: 23236492) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

I am very pleased to hear you would like to accept our offer and test ab60272 in ELISA on Rhesus Monkey. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for ELISA on Rhesus Monkeyand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Question
Answer

Thank you for your calls last week and for your patience while I have worked to have this peptide added to our catalog. The peptide is now available as ab115238- https://www.abcam.com/intestinal-FABP-peptide-ab115238.html Please let me know if there is anything else that we can do for you.

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