Key features and details
- Goat polyclonal to I-FABP
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-I-FABP antibody
See all I-FABP primary antibodies
DescriptionGoat polyclonal to I-FABP
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Rhesus monkey
- WB: Human Duodenum lysate. IHC-P: Human small intestine.
Previously labelled as intestinal FABP.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
Our Abpromise guarantee covers the use of ab60272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.001 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 15 kDa).
A 1 hour primary incubation is recommended for this product.
|IHC-P||Use a concentration of 2 - 3 µg/ml.|
FunctionFABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
Tissue specificityExpressed in the small intestine and at much lower levels in the large intestine. Highest expression levels in the jejunum.
Sequence similaritiesBelongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
DomainForms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
- Information by UniProt
- FABP 2 antibody
- FABP2 antibody
- FABPI antibody
ab60272 antibody (0.001µg/ml) staining of I-FABP in Human Duodenum lysate (35µg protein in RIPA buffer)
Predicted band size: 15 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
ab60272 staining I-FABP in Human small intestine by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue sections underwent antigen retrieval by steam with citrate buffer pH 6.0. The AP staining procedure was used for detection.
ab60272 has been referenced in 2 publications.
- Ziegler AL et al. Epithelial restitution defect in neonatal jejunum is rescued by juvenile mucosal homogenate in a pig model of intestinal ischemic injury and repair. PLoS One 13:e0200674 (2018). PubMed: 30138372
- Treutlein G et al. Novel potential interacting partners of fibronectin in spontaneous animal model of interstitial cystitis. PLoS One 7:e51391 (2012). WB, ICC/IF ; Cat . PubMed: 23236492