Anti-iNOS antibody [K13-A] (ab136918)
Key features and details
- Rabbit monoclonal [K13-A] to iNOS
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-iNOS antibody [K13-A]
See all iNOS primary antibodies -
Description
Rabbit monoclonal [K13-A] to iNOS -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to a region of Human iNOS.
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Epitope
Antibody recognizes the epitope located between Ser1118 - Gly1129 -
Positive control
- Mouse brain tissue lysate - total protein (ab30151) can be used as a positive control in WB. Raw264.7 whole cell lysate (LPS treated)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 8.00
Preservative: 0.05% Sodium azide
Constituents: 0.32% Tris HCl, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This immunoglobulin is the product of one single B-cell line from the crude anti-peptide polyclonal anti-serum. This antibody is purified using a proprietary technique and offers a completely post-translationally modified and properly glycosylated antibody. This offers increased stability. -
Clonality
Monoclonal -
Clone number
K13-A -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab136918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/2000. Predicted molecular weight: 131 kDa. Incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature.
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Notes |
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WB
1/2000. Predicted molecular weight: 131 kDa. Incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature. |
Target
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Function
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. -
Tissue specificity
Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. -
Sequence similarities
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain. - Information by UniProt
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Database links
- Entrez Gene: 18126 Mouse
- Entrez Gene: 24599 Rat
- SwissProt: P29477 Mouse
- SwissProt: Q06518 Rat
- Unigene: 2893 Mouse
- Unigene: 10400 Rat
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Alternative names
- HEP-NOS antibody
- Hepatocyte NOS antibody
- HEPNOS antibody
see all
Images
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All lanes : Anti-iNOS antibody [K13-A] (ab136918) at 1/2000 dilution
Lane 1 : Raw264.7 Whole Cell Lysate
Lane 2 : Raw264.7 Whole Cell Lysate (LPS stimulated)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anit Rabbit IR680 at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 131 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa, 36 kDa, 42 kDa, 50 kDa. We are unsure as to the identity of these extra bands.This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab136918 overnight at 4°C. Antibody binding was detected using Goat anti Mouse IR680 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx
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Anti-iNOS antibody [K13-A] (ab136918) at 1/2000 dilution + iNOS Overexpression Lysate (Human) at 0.1 µg
Secondary
Goat anti Rabbit IR680 at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 131 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab136918 overnight at 4°C. Antibody binding was detected using Goat anti Rabbit IR680 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx
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Anti-iNOS antibody [K13-A] (ab136918) at 1/2000 dilution + Mouse brain lysate at 50 µg
Predicted band size: 131 kDa
Datasheets and documents
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Datasheet download
References (18)
ab136918 has been referenced in 18 publications.
- Naeem AG et al. Nebivolol elicits a neuroprotective effect in the cuprizone model of multiple sclerosis in mice: emphasis on M1/M2 polarization and inhibition of NLRP3 inflammasome activation. Inflammopharmacology 30:2197-2209 (2022). PubMed: 35948811
- Saeed MEM et al. Biomarker Expression Profiling in Cervix Carcinoma Biopsies Unravels WT1 as a Target of Artesunate. Cancer Genomics Proteomics 19:727-739 (2022). PubMed: 36316038
- Fidilio A et al. Prenatal stress induces a depressive-like phenotype in adolescent rats: The key role of TGF-β1 pathway. Front Pharmacol 13:1075746 (2022). PubMed: 36532726
- Yan J et al. Rhoifolin Ameliorates Osteoarthritis via Regulating Autophagy. Front Pharmacol 12:661072 (2021). PubMed: 34122080
- Kim KJ et al. SR-5, the specific ratio of Korean multi-herbal formula: An evaluation of antiulcerogenic effects on experimentally induced gastric ulcers in mice. Dose Response 19:15593258211044329 (2021). PubMed: 34690616