Anti-iNOS antibody [NOS-IN] (ab49999)
Key features and details
- Mouse monoclonal [NOS-IN] to iNOS
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG1
Overview
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Product name
Anti-iNOS antibody [NOS-IN]
See all iNOS primary antibodies -
Description
Mouse monoclonal [NOS-IN] to iNOS -
Host species
Mouse -
Specificity
This antibody reacts specifically with inducible-type nitricoxide synthase (iNOS, also termed macNOS and mNOS). It does not react with NOS derived from brain.
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Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide corresponding to Mouse iNOS aa 1126-1144 conjugated to keyhole limpet haemocyanin.
Sequence:FSYGAKKGSALEEPKATRL
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Positive control
- WB: Whole cell extract of RAW 264.7 activated with LPS and IFN-gamma. ICC/IF: RAW 264.7 activated with LPS and IFN-gamma.
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General notes
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.087% Sodium azide
Constituent: 2.93% PBS -
Concentration information loading...
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Purification notes
This antibody is purified from hybridoma cell culture. -
Clonality
Monoclonal -
Clone number
NOS-IN -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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ELISA pair antibody
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab49999 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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WB | (4) |
Use at an assay dependent concentration. Predicted molecular weight: 131 kDa.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 131 kDa. |
Target
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Function
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. -
Tissue specificity
Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. -
Sequence similarities
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain. - Information by UniProt
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Database links
- Entrez Gene: 18126 Mouse
- SwissProt: P29477 Mouse
- Unigene: 2893 Mouse
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Alternative names
- HEP-NOS antibody
- Hepatocyte NOS antibody
- HEPNOS antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence analysis of IFN-γ/LPS-activated RAW-264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling iNOS with ab49999 at 1/2000 dilution.
Cells were fixed and permeabilized with 4% paraformaldehyde and 0.5% Triton™ X-100. The antibody was developed using Goat anti-Mouse IgG, Cy3-conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
This image was generated using the ascites version of the product.
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Lane 1 : Anti-iNOS antibody [NOS-IN] (ab49999) at 1/500 dilution
Lane 2 : Anti-iNOS antibody [NOS-IN] (ab49999) at 1/1000 dilution
Lane 3 : Anti-iNOS antibody [NOS-IN] (ab49999) at 1/2500 dilution
Lane 4 : Anti-iNOS antibody [NOS-IN] (ab49999) at 1/5000 dilution
All lanes : IFN-gamma/LPS-activated RAW-264.7 cell extract
Secondary
All lanes : Goat Anti-Mouse IgG-Peroxidase and chemiluminescent substrate
Predicted band size: 131 kDaThis image was generated using the ascites version of the product.
Datasheets and documents
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SDS download
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Datasheet download
References (79)
ab49999 has been referenced in 79 publications.
- Ma Y et al. Photobiomodulation Attenuates Neurotoxic Polarization of Macrophages by Inhibiting the Notch1-HIF-1α/NF-κB Signalling Pathway in Mice With Spinal Cord Injury. Front Immunol 13:816952 (2022). PubMed: 35371065
- Fan Z et al. Intestinal Flora Changes Induced by a High-Fat Diet Promote Activation of Primordial Follicles through Macrophage Infiltration and Inflammatory Factor Secretion in Mouse Ovaries. Int J Mol Sci 23:N/A (2022). PubMed: 35563189
- Fan H et al. Exosomes derived from olfactory ensheathing cells provided neuroprotection for spinal cord injury by switching the phenotype of macrophages/microglia. Bioeng Transl Med 7:e10287 (2022). PubMed: 35600663
- Hang Z et al. Iguratimod Attenuates Macrophage Polarization and Antibody-Mediated Rejection After Renal Transplant by Regulating KLF4. Front Pharmacol 13:865363 (2022). PubMed: 35614941
- Carrascal L et al. Acute Colon Inflammation Triggers Primary Motor Cortex Glial Activation, Neuroinflammation, Neuronal Hyperexcitability, and Motor Coordination Deficits. Int J Mol Sci 23:N/A (2022). PubMed: 35628158