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    iars2-antibody-ab242116.pdf

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Epigenetics and Nuclear Signaling DNA / RNA Translation tRNA synthetase
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Anti-IARS2 antibody (ab242116)

  • Datasheet
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Abpromise

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Western blot - Anti-IARS2 antibody (ab242116)
  • Immunoprecipitation - Anti-IARS2 antibody (ab242116)

Key features and details

  • Rabbit polyclonal to IARS2
  • Suitable for: IP, WB
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-IARS2 antibody
    See all IARS2 primary antibodies
  • Description

    Rabbit polyclonal to IARS2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human IARS2 aa 962-1012. The exact sequence is proprietary.
    Database link: Q9NSE4

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: HEK-293T whole cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibody was affinity purified using an epitope specific to IARS2 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • tRNA synthetase

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)

Applications

Our Abpromise guarantee covers the use of ab242116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.

20 µl/mg lysate

WB 1/1000. Predicted molecular weight: 114 kDa.

Target

  • Sequence similarities

    Belongs to the class-I aminoacyl-tRNA synthetase family.
  • Cellular localization

    Mitochondrion matrix.
  • Target information above from: UniProt accession Q9NSE4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 55699 Human
    • Omim: 612801 Human
    • SwissProt: Q9NSE4 Human
    • Unigene: 262823 Human
    • Alternative names

      • 2010002H18Rik antibody
      • C79125 antibody
      • EC 6.1.1.5 antibody
      • FLJ10326 antibody
      • Iars2 antibody
      • IleRS antibody
      • isoleucine tRNA ligase 2, mitochondrial antibody
      • isoleucine tRNA ligase, mitochondrial antibody
      • isoleucine tRNA synthetase 2, mitochondrial antibody
      • Isoleucine--tRNA ligase antibody
      • isoleucine-tRNA synthetase 2 antibody
      • isoleucyl tRNA synthetase 2, mitochondrial antibody
      • Isoleucyl-tRNA synthetase antibody
      • MGC63429 antibody
      • mitochondrial antibody
      • mitochondrial isoleucine tRNA synthetase antibody
      • rCG_20084 antibody
      • RGD1311857 antibody
      • SYIM_HUMAN antibody
      see all

    Images

    • Western blot - Anti-IARS2 antibody (ab242116)
      Western blot - Anti-IARS2 antibody (ab242116)
      All lanes : Anti-IARS2 antibody (ab242116) at 1/1000 dilution

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

      Lysates/proteins at 15 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 114 kDa


      Exposure time: 30 seconds


      Prepared using NETN lysis buffer.

    • Immunoprecipitation - Anti-IARS2 antibody (ab242116)
      Immunoprecipitation - Anti-IARS2 antibody (ab242116)

      IARS2 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
      ab242116 used for IP at 20 µl per reaction. For WB at 1/100 dilution.

      Lane 1: ab242116 IP in HEK-293T whole cell lysate.
      Lane 2: Control IgG IP in HEK-293T whole cell lysate.

      Chemiluminescence detection: 30 seconds.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab242116? Please let us know so that we can cite the reference in this datasheet.

    ab242116 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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