Anti-IARS2 antibody (ab242116)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-IARS2 antibody
See all IARS2 primary antibodies -
Description
Rabbit polyclonal to IARS2 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human IARS2 aa 962-1012. The exact sequence is proprietary.
Database link: Q9NSE4 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: HEK-293T whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to IARS2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab242116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. 20 µl/mg lysate |
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| WB | 1/1000. Predicted molecular weight: 114 kDa. |
Target
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Sequence similarities
Belongs to the class-I aminoacyl-tRNA synthetase family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 55699 Human
- Omim: 612801 Human
- SwissProt: Q9NSE4 Human
- Unigene: 262823 Human
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Alternative names
- 2010002H18Rik antibody
- C79125 antibody
- EC 6.1.1.5 antibody
see all
Images
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Western blot - Anti-IARS2 antibody (ab242116)All lanes : Anti-IARS2 antibody (ab242116) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 114 kDa
Exposure time: 30 secondsPrepared using NETN lysis buffer.
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Immunoprecipitation - Anti-IARS2 antibody (ab242116)IARS2 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab242116 used for IP at 20 µl per reaction. For WB at 1/100 dilution.Lane 1: ab242116 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Chemiluminescence detection: 30 seconds.
Datasheets and documents
References
ab242116 has not yet been referenced specifically in any publications.
