Product nameAnti-IARS2 antibody
See all IARS2 primary antibodies
DescriptionRabbit polyclonal to IARS2
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human IARS2 aa 962-1012. The exact sequence is proprietary.
Database link: Q9NSE4
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to IARS2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab242116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
20 µl/mg lysate
|WB||1/1000. Predicted molecular weight: 114 kDa.|
Sequence similaritiesBelongs to the class-I aminoacyl-tRNA synthetase family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- 2010002H18Rik antibody
- C79125 antibody
- EC 220.127.116.11 antibody
All lanes : Anti-IARS2 antibody (ab242116) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 114 kDa
Exposure time: 30 seconds
Prepared using NETN lysis buffer.
IARS2 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab242116 used for IP at 20 µl per reaction. For WB at 1/100 dilution.
Lane 1: ab242116 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Chemiluminescence detection: 30 seconds.
ab242116 has not yet been referenced specifically in any publications.