Key features and details
- Rabbit polyclonal to IARS2
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IARS2 antibody
See all IARS2 primary antibodies
DescriptionRabbit polyclonal to IARS2
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human IARS2 aa 962-1012. The exact sequence is proprietary.
Database link: Q9NSE4
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: HEK-293T whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to IARS2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab242116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
20 µl/mg lysate
|WB||1/1000. Predicted molecular weight: 114 kDa.|
Sequence similaritiesBelongs to the class-I aminoacyl-tRNA synthetase family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- 2010002H18Rik antibody
- C79125 antibody
- EC 18.104.22.168 antibody
All lanes : Anti-IARS2 antibody (ab242116) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 114 kDa
Exposure time: 30 seconds
Prepared using NETN lysis buffer.
IARS2 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab242116 used for IP at 20 µl per reaction. For WB at 1/100 dilution.
Lane 1: ab242116 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Chemiluminescence detection: 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab242116 has not yet been referenced specifically in any publications.