• Product name
  • Description
    Rabbit polyclonal to IB-1
  • Host species
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse IB-1 (N terminal).

  • Positive control
    • Mouse Intestine. Rat kidney. Jurkat cell lysate.
  • General notes

    Protein previously labeled as JIP1.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Thimerosal (merthiolate)
    Constituents: PBS, 50% Glycerol, 0.1% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab24449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa). A band of 110 kDa can also be observed in human samples.


  • Relevance
    Function: The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Required for JNK activation in response to excitotoxic stress. Cytoplasmic MAPK8IP1 causes inhibition of JNK-regulated activity by retaining JNK in the cytoplasm and inhibiting JNK phosphorylation of c-Jun. May also participate in ApoER2-specific reelin signaling. Directly, or indirectly, regulates GLUT2 gene expression and beta-cell function. Appears to have a role in cell signaling in mature and developing nerve terminals. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins (By similarity). Functions as an anti-apoptotic protein and whose level seems to influence the beta-cell death or survival response. Tissue specificity: Highly expressed in brain. Expressed in neurons, localizing to neurite tips in differentiating cells. Also expressed in the pancreas, testis and prostate. Low levels in heart, ovary and small intestine. Decreased levels in pancreatic beta cells sensitize cells to IL-1-beta-induced apoptosis. Disease: Diabetes mellitus, non-insulin-dependent Similarity: Belongs to the JIP scaffold family. Contains 1 PID domain. Contains 1 SH3 domain. Domain: The destruction boxes (D-box) may act as recognition signals for degradation via the ubiquitin-proteasome pathway. A minimal inhibitory domain prevents pancreatic beta cell apoptosis in vitro, and prevents activation of c-jun by MAPK8, MAPK9 and MAPK10. The SH3 domain mediates homodimerization. PTM: Phosphorylated by MAPK8, MAPK9 and MAPK10. Phosphorylation on Thr-103 is also necessary for the dissociation and activation of MAP3K12. Phosphorylated by isoform 1 and isoform 2 of VRK2. Hyperphosphorylated during mitosis following activation of stress-activated and MAP kinases. Ubiquitinated. Two preliminary events are required to prime for ubiquitination; phosphorylation and an increased in intracellular calcium concentration. Then, the calcium influx initiates ubiquitination and degradation by the ubiquitin-proteasome pathway.
  • Cellular localization
    Cytoplasmic. Accumulates in cell surface projections. Under certain stress conditions, translocates to the perinuclear region of neurons. In insulin-secreting cells, detected in both the cytoplasm and nucleus
  • Database links
  • Alternative names
    • C jun amino terminal kinase interacting protein 1 antibody
    • C-jun-amino-terminal kinase-interacting protein 1 antibody
    • IB 1 antibody
    • IB-1 antibody
    • IB1 antibody
    • Islet brain 1 antibody
    • Islet-brain 1 antibody
    • JIP 1 antibody
    • JIP-1 antibody
    • JIP1_HUMAN antibody
    • JNK interacting protein 1 antibody
    • JNK MAP kinase scaffold protein 1 antibody
    • JNK-interacting protein 1 antibody
    • MAPK8IP 1 antibody
    • MAPK8IP1 antibody
    • MAPK8IP1 mitogen-activated protein kinase 8 interacting protein 1 antibody
    • Mitogen activated protein kinase 8 interacting protein 1 antibody
    • Mitogen-activated protein kinase 8-interacting protein 1 antibody
    • PRKM8 antibody
    • PRKM8 interacting protein antibody
    • PRKM8IP antibody
    see all


  • All lanes : Anti-IB-1 antibody (ab24449)

    Lane 1 : Rat kidney
    Lane 2 : Mouse Intestine
    Lane 3 : Jurkat Cell lysate

    Predicted band size: 78 kDa
    Observed band size: 78 kDa
    Additional bands at: 110 kDa (possible isoform)

  • ICC/IF image of ab24449 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24449, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Sherman MA  et al. Soluble Conformers of Aß and Tau Alter Selective Proteins Governing Axonal Transport. J Neurosci 36:9647-58 (2016). Read more (PubMed: 27629715) »

See 1 Publication for this product

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Thank you for your enquiry. To our knowledge, the antibody has yet to be tested in immunoprecipitation. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, we encourage you to s...

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