Overview

  • Product name

    Anti-Iba1 antibody [1022-5] (FITC)
    See all Iba1 primary antibodies
  • Description

    Mouse monoclonal [1022-5] to Iba1 (FITC)
  • Host species

    Mouse
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Specificity

    Specific for activated macrophages.
  • Tested applications

    Suitable for: Flow Cytmore details
    Unsuitable for: IHC
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human Iba1.
    Database link: P55008

  • Positive control

    • Flow Cytometry: THP-1 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab15691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.

ab91368 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC.
  • Target

    • Function

      Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
    • Tissue specificity

      Detected in T-lymphocytes and peripheral blood mononuclear cells.
    • Sequence similarities

      Contains 2 EF-hand domains.
    • Post-translational
      modifications

      Phosphorylated on serine residues.
    • Cellular localization

      Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.
    • Information by UniProt
    • Database links

    • Alternative names

      • AIF 1 antibody
      • AIF-1 antibody
      • Aif1 antibody
      • AIF1 protein antibody
      • AIF1_HUMAN antibody
      • Allograft inflammatory factor 1 antibody
      • Allograft inflammatory factor 1 splice variant G antibody
      • allograft inflammatory factor-1 splice variant Hara-1 antibody
      • balloon angioplasty responsive transcription antibody
      • BART 1 antibody
      • G1 antibody
      • G1 putative splice variant of allograft inflamatory factor 1 antibody
      • IBA 1 antibody
      • IBA1 antibody
      • interferon gamma responsive transcript antibody
      • Interferon responsive transcript 1 antibody
      • interferon responsive transcript factor 1 antibody
      • Ionized calcium binding adapter molecule 1 antibody
      • Ionized calcium-binding adapter molecule 1 antibody
      • ionized calcium-binding adapter molecule antibody
      • IRT 1 antibody
      • IRT1 antibody
      • Microglia response factor antibody
      • MRF1 antibody
      • Protein g1 antibody
      see all

    Images

    • Overlay histogram showing THP-1 (human monocytic leukemia cell line) cells stained with ab15691 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15691, 10 µl/1 x 106 cells) for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG2b (FITC) (ab91368, 10 µl/1 x 106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP-1 cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    References

    This product has been referenced in:

    • Trkov Bobnar S  et al. Fingolimod Suppresses the Proinflammatory Status of Interferon-?-Activated Cultured Rat Astrocytes. Mol Neurobiol N/A:N/A (2019). Read more (PubMed: 30701416) »
    • Lei J  et al. Maternal dendrimer-based therapy for inflammation-induced preterm birth and perinatal brain injury. Sci Rep 7:6106 (2017). Flow Cyt ; Mouse . Read more (PubMed: 28733619) »
    See all 6 Publications for this product

    Customer reviews and Q&As

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    1-2 of 2 Abreviews

    Application
    Flow Cytometry
    Sample
    Mouse Cell (Brain)
    Specification
    Brain
    Fixation
    Paraformaldehyde
    Permeabilization
    Yes - BD Perm/Wash
    Gating Strategy
    Microglia

    Abcam user community

    Verified customer

    Submitted Feb 01 2012

    Application
    Flow Cytometry
    Sample
    Mouse Cell (spinal cord neuron)
    Specification
    spinal cord neuron
    Preparation
    Cell harvesting/tissue preparation method: primary cell culture
    Sample buffer: 0.5% BSA + 2 mM EDTA/PBS without divalent cations
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    macrophages

    Ms. Minjung Kwon

    Verified customer

    Submitted Jan 21 2010

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