Key features and details
- Goat polyclonal to Iba1
- Suitable for: IHC-P
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-Iba1 antibody
See all Iba1 primary antibodies
DescriptionGoat polyclonal to Iba1
SpecificityIf looking for a monoclonal anti-Iba1 alternative we can recommend our RabMAb ab178846. This has the same immunogen as ab5076. In recent testing in IHC-P this antibody only worked in Rat tissue, therefore we have removed Human and mouse as a "does not react with" species. However, feedback from our customers and publications suggest that this antibody recognizes human and mouse. Nevertheless, we cannot guarantee this antibody in these species.
Tested Applications & Species
Application Species IHC-PRat
Synthetic peptide corresponding to Rat Iba1 aa 100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 3% BSA
This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us.
Concentration information loading...
PurityImmunogen affinity purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab107159 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Use at an assay dependent concentration.
Use at an assay dependent concentration.
FunctionActin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
Tissue specificityDetected in T-lymphocytes and peripheral blood mononuclear cells.
Sequence similaritiesContains 2 EF-hand domains.
modificationsPhosphorylated on serine residues.
Cellular localizationCytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.
- Information by UniProt
- AIF 1 antibody
- AIF-1 antibody
- Aif1 antibody
IHC image of Iba1 staining in a section of formalin-fixed paraffin-embedded normal rat brain performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab107159, 5ug/ml, for 15 mins at room temperature. An HRP-conjugated donkey anti-goat IgG secondary was used for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
IHC-P image of Iba1 staining on marmoset (common) kidney sections using ab107159 (1:5000). The image shows macrophage infiltration in the cortex. The paraffin embedded sectons were subjected to heat mediated antigen retrieval using citric acid. The sections was then blocked using 1% BSA at 21°C for 10 min. The primary antibody was incubated at 21°C for 16 hours. The secondary antibody was rabbit polyclonal to sheep IgG conjugated to biotin at 1:200.
ab107159 staining Iba1 in Rat liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 15 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2000 in PBS + 1% BSA) for 1 hour at 20°C. An undiluted HRP-conjugated anti-goat IgG polyclonal was used as the secondary antibody. Kupffer cells are positive for Iba-1.
IHC-P image of Iba1 staining on rat spleen sections using ab107159 (1:2000). The paraffin embedded sectons were subjected to heat mediated antigen retrieval using citric acid. The sections was then blocked using 1% BSA at 21°C for 10 min. The primary antibody was incubated at 21°C for 2 hours. The secondary antibody was goat polyclonal to anti-sheep conjugated to biotin at 1:250.
ab107159 has been referenced in 90 publications.
- Caron NS et al. Potent and sustained huntingtin lowering via AAV5 encoding miRNA preserves striatal volume and cognitive function in a humanized mouse model of Huntington disease. Nucleic Acids Res 48:36-54 (2020). PubMed: 31745548
- Evonuk KS et al. Reduction of AMPA receptor activity on mature oligodendrocytes attenuates loss of myelinated axons in autoimmune neuroinflammation. Sci Adv 6:eaax5936 (2020). PubMed: 31934627
- Stegmayr C et al. Investigation of Cerebral O-(2-[18F]Fluoroethyl)-L-Tyrosine Uptake in Rat Epilepsy Models. Mol Imaging Biol N/A:N/A (2020). PubMed: 32409931
- Estrada SM et al. The orphan nuclear receptor Nr4a1 mediates perinatal neuroinflammation in a murine model of preterm labor. Cell Death Dis 11:11 (2020). PubMed: 31907354
- Dobolyi A et al. Exclusive neuronal detection of KGDHC-specific subunits in the adult human brain cortex despite pancellular protein lysine succinylation. Brain Struct Funct 225:639-667 (2020). PubMed: 31982949