Immunocytochemistry/ Immunofluorescence abreview for Anti-Iba1 antibody

Average
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (glioblastoma cell line C6)
Specification
glioblastoma cell line C6
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,1% Triton X100 in PBS
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C

Other product details

Dilution
1/50
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0,5% BSA in PBS

Secondary antibody

Name
Non-Abcam antibody was used: rabbit anti goat (C2821)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/120

Additional data

Additional Notes
Nucleii are counterstained with DAPI

Abcam user community

Verified customer

Submitted Jan 24 2012

Sign up