Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Iba1 antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Rat Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: anti Sheep/Goat IgG
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200

Additional data

Additional Notes
NB: sheep and goat antibodies cross-react thus it is quite proper to use an anti sheep antibody when demonstrating a goat primary antibody, under these conditions.

Mr. Carl Hobbs

Verified customer

Submitted Dec 18 2013

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