Western blot abreview for Anti-Iba1 antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Hippocampus)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
40 µg
Specification
Hippocampus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Donkey anti-goat IgG-HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
45 second(s)
Bands
Specific: 17-18 kDa

Dr. Hyeon Joo Ham

Verified customer

Submitted Oct 23 2018

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