Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Iba1 antibody

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Abreviews
This product is known to not work in this application or species.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (spinal dorsal horn)
Antigen retrieval step
None
Permeabilization
Yes - TritonX(0.1%),Tween(0.05%)
Specification
spinal dorsal horn
Blocking step
PBS with 3% BSA and 10 % Donkey serum as blocking agent for 2 hour(s) and 0 minute(s) · Temperature: 20°C
Fixative
Formaldehyde

Other product details

Incubation time
48 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS with 3%BSA
Dilution
1/100

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Donkey anti-Goat IgG (H+L) Alexa Fluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

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Submitted May 17 2019

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