Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Iba1 antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (brain)
Antigen retrieval step
None
Permeabilization
Yes - tritonX 0.05%
Specification
brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/200

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: donkey anti goat alexa
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594

Additional data

Additional Notes
blue is cell nuclei

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Submitted May 21 2019

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