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    iba1-antibody-epr16589-ab178847.pdf

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RecombinantRabMAb

Recombinant Anti-Iba1 antibody [EPR16589] (ab178847)

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  • SDS
  • Certificate of Compliance
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16589] (ab178847)
  • Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody [EPR16589] (ab178847)
  • Immunoprecipitation - Anti-Iba1 antibody [EPR16589] (ab178847)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16589] (ab178847)
  • Immunohistochemistry (PFA fixed) - Anti-Iba1 antibody [EPR16589] (ab178847)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16589] (ab178847)
  • Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody [EPR16589] (ab178847)
  • Western blot - Anti-Iba1 antibody [EPR16589] (ab178847)
  • Western blot - Anti-Iba1 antibody [EPR16589] (ab178847)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16589] (ab178847)
  • Western blot - Anti-Iba1 antibody [EPR16589] (ab178847)
  • Western blot - Anti-Iba1 antibody [EPR16589] (ab178847)
  • Anti-Iba1 antibody [EPR16589] (ab178847)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16589] to Iba1
  • Suitable for: IHC (PFA fixed), IHC-P, WB, IP, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free HRP

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Overview

  • Product name

    Anti-Iba1 antibody [EPR16589]
    See all Iba1 primary antibodies
  • Description

    Rabbit monoclonal [EPR16589] to Iba1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC (PFA fixed), IHC-P, WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human spleen lysate; THP-1, MOLT-4 and U937 whole cell lysates; Mouse and rat spleen and testis lysates; Mouse hippocampus and brain lysates. IHC-P: Human cerebrum, mouse endometrium and rat cerebrum tissues. ICC/IF: U937 and THP-1 cells. IP: Mouse spleen whole cell lysate. IHC-Fr: Mouse hippocampus tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16589
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Other
    • Immunology
    • Immune Interventions
    • Transplantation
    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Microglia marker
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • HRP Anti-Iba1 antibody [EPR16589] (ab216544)
    • Anti-Iba1 antibody [EPR16589] - BSA and Azide free (ab221790)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Iba1 protein (ab105593)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • VeriBlot for IP Detection Reagent (HRP) (ab131366)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab178847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC (PFA fixed)
Use at an assay dependent concentration.
IHC-P (7)
1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB (3)
1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IP
1/40.
ICC/IF (3)
1/100.
Notes
IHC (PFA fixed)
Use at an assay dependent concentration.
IHC-P
1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IP
1/40.
ICC/IF
1/100.

Target

  • Function

    Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
  • Tissue specificity

    Detected in T-lymphocytes and peripheral blood mononuclear cells.
  • Sequence similarities

    Contains 2 EF-hand domains.
  • Post-translational
    modifications

    Phosphorylated on serine residues.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.
  • Target information above from: UniProt accession P55008 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 199 Human
    • Entrez Gene: 11629 Mouse
    • Entrez Gene: 29427 Rat
    • Omim: 601833 Human
    • SwissProt: P55008 Human
    • SwissProt: O70200 Mouse
    • SwissProt: P55009 Rat
    • Unigene: 76364 Human
    • Unigene: 10747 Mouse
    • Unigene: 32080 Rat
    see all
  • Alternative names

    • AIF 1 antibody
    • AIF-1 antibody
    • Aif1 antibody
    • AIF1 protein antibody
    • AIF1_HUMAN antibody
    • Allograft inflammatory factor 1 antibody
    • Allograft inflammatory factor 1 splice variant G antibody
    • allograft inflammatory factor-1 splice variant Hara-1 antibody
    • balloon angioplasty responsive transcription antibody
    • BART 1 antibody
    • G1 antibody
    • G1 putative splice variant of allograft inflamatory factor 1 antibody
    • IBA 1 antibody
    • IBA1 antibody
    • interferon gamma responsive transcript antibody
    • Interferon responsive transcript 1 antibody
    • interferon responsive transcript factor 1 antibody
    • Ionized calcium binding adapter molecule 1 antibody
    • Ionized calcium-binding adapter molecule 1 antibody
    • ionized calcium-binding adapter molecule antibody
    • IRT 1 antibody
    • IRT1 antibody
    • Microglia response factor antibody
    • MRF1 antibody
    • Protein g1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16589] (ab178847)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16589] (ab178847)Stephanie R. Beldick et al.,PLOS ONE.,Fig 5.; doi.org/10.1371/journal.pone.0208105.

    Slides were washed with 1x PBS and then blocked in 5% goat serum containing 0.3% Triton X-100. Iba-1 was stained using ab178847 at 1/500 dilution in immunohistochemical analysis. Representative immunofluorescence is depicted for KO Sham (F top left), KO injured (F bottom left), WT Sham (F top right), and WT injured (F bottom right) in the right and left hemispheres (blue = DAPI, green = Iba-1). Scale bars = 100μm, 20x. HI = hypoxic-ischemic brain injury.

    Area occupied by Iba-1-positive staining was elevated in KO injured animals when compared to uninjured controls, while stain area was not elevated in WT injured animals 

  • Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody [EPR16589] (ab178847)
    Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody [EPR16589] (ab178847)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized U937 (Human histiocytic lymphoma cell line) cells labeling Iba1 with ab178847 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on U937 cell line.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab178847 at 1/100 dilution followed by ab150120 at 1/1000 dilution.

    -ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

  • Immunoprecipitation - Anti-Iba1 antibody [EPR16589] (ab178847)
    Immunoprecipitation - Anti-Iba1 antibody [EPR16589] (ab178847)

    Iba1 was immunoprecipitated from 1mg of Mouse spleen whole cell lysate with ab178847 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab178847 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse spleen whole cell lysate 10µg (Input).

    Lane 2: ab178847 IP in Mouse spleen whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal-Isotype Control (ab172730) instead of ab178847 in Mouse spleen whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16589] (ab178847)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16589] (ab178847)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Iba1 with ab178847 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasm staining on microglia of the normal Human cerebrum is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

  • Immunohistochemistry (PFA fixed) - Anti-Iba1 antibody [EPR16589] (ab178847)
    Immunohistochemistry (PFA fixed) - Anti-Iba1 antibody [EPR16589] (ab178847)

    Iba1 antibody ab178847 was used with Tissue Clearing Kit ab243298 to penetrate, stain and clear a 1 mm coronal section of mouse brain. Blue: DAPI , Green: Iba1.

    Learn more about tissue clearing kits, reagents, and protocols designed to make it easier to stain thick tissue sections and get more data from each valuable tissue section.

    For 1 mm brain sections, we recommend a starting dilution of 1:100, and also using Goat Anti-Rabbit IgG H&L AlexaFluor488 (ab150077) at a dilution of 1:400.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16589] (ab178847)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16589] (ab178847)

    Immunohistochemical analysis of paraffin-embedded Mouse endometrium tissue labeling Iba1 with ab178847 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasm staining on macrophages of the mouse endometrium.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody [EPR16589] (ab178847)
    Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody [EPR16589] (ab178847)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized THP-1 (Human monocytic leukemia cell line) cells labeling Iba1 with ab178847 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on THP-1 cell line.

    The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab178847 at 1/100 dilution followed by ab150120 at 1/1000 dilution.

    -ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

  • Western blot - Anti-Iba1 antibody [EPR16589] (ab178847)
    Western blot - Anti-Iba1 antibody [EPR16589] (ab178847)
    All lanes : Anti-Iba1 antibody [EPR16589] (ab178847) at 1/2000 dilution

    Lane 1 : Human spleen lysate
    Lane 2 : THP-1 (Human monocytic leukemia cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Iba1 antibody [EPR16589] (ab178847)
    Western blot - Anti-Iba1 antibody [EPR16589] (ab178847)
    Different batches of ab178847 were tested on U-937 (Human histiocytic lymphoma monocyte) lysate at 0.5 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 17 kDa.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16589] (ab178847)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16589] (ab178847)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Iba1 with ab178847 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasm staining on microglia of the rat cerebrum is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Iba1 antibody [EPR16589] (ab178847)
    Western blot - Anti-Iba1 antibody [EPR16589] (ab178847)
    All lanes : Anti-Iba1 antibody [EPR16589] (ab178847) at 1/1000 dilution

    Lane 1 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate
    Lane 2 : U937 (Human histiocytic lymphoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Iba1 antibody [EPR16589] (ab178847)
    Western blot - Anti-Iba1 antibody [EPR16589] (ab178847)
    All lanes : Anti-Iba1 antibody [EPR16589] (ab178847) at 1/1000 dilution

    Lane 1 : Mouse spleen lysate
    Lane 2 : Rat spleen lysate
    Lane 3 : Mouse testis lysate
    Lane 4 : Rat testis lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1 and 2: 1 minute; Lane 3 and 4: 3 minutes.

  • Anti-Iba1 antibody [EPR16589] (ab178847)
    Anti-Iba1 antibody [EPR16589] (ab178847)

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (142)

Publishing research using ab178847? Please let us know so that we can cite the reference in this datasheet.

ab178847 has been referenced in 142 publications.

  • Li T  et al. L-Cysteine Provides Neuroprotection of Hypoxia-Ischemia Injury in Neonatal Mice via a PI3K/Akt-Dependent Mechanism. Drug Des Devel Ther 15:517-529 (2021). PubMed: 33603342
  • Deng S  et al. Recombinant CCL17-dependent CCR4 activation alleviates neuroinflammation and neuronal apoptosis through the PI3K/AKT/Foxo1 signaling pathway after ICH in mice. J Neuroinflammation 18:62 (2021). PubMed: 33648537
  • He X  et al. Apigenin Nanoparticle Attenuates Renal Ischemia/Reperfusion Inflammatory Injury by Regulation of miR-140-5p/CXCL12/NF-?B Signaling Pathway. J Biomed Nanotechnol 17:64-77 (2021). PubMed: 33653497
  • Wang L  et al. Neuroprotective effect of ketamine against TNF-a-induced necroptosis in hippocampal neurons. J Cell Mol Med 25:3449-3459 (2021). PubMed: 33660415
  • Doroshenko ER  et al. Peroxisome Proliferator-Activated Receptor-d Deficiency in Microglia Results in Exacerbated Axonal Injury and Tissue Loss in Experimental Autoimmune Encephalomyelitis. Front Immunol 12:570425 (2021). PubMed: 33732230
View all Publications for this product

Customer reviews and Q&As

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1-10 of 17 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Iba1 antibody [EPR16589]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: buffer pH9
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 03 2022

Immunocytochemistry/ Immunofluorescence abreview for Anti-Iba1 antibody [EPR16589]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (organoid)
Permeabilization
Yes - 0.3%tritonX-100
Specification
organoid
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 18 2022

Immunocytochemistry/ Immunofluorescence abreview for Anti-Iba1 antibody [EPR16589]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (brain)
Permeabilization
Yes - 0.3%tritonX-100
Specification
brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 18 2022

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Iba1 antibody [EPR16589]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Syrian hamster Cell (Brain tissue)
Permeabilization
Yes - 1% Triton X-100 in PBS
Specification
Brain tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 30 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Iba1 antibody [EPR16589]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (lymph node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Leica ER2
Permeabilization
No
Specification
lymph node
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 08 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Iba1 antibody [EPR16589]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH9
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 23°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Victor Bernal

Verified customer

Submitted Nov 09 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Iba1 antibody [EPR16589]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Specification
Brain
Blocking step
(agent) for 30 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 31 2020

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Iba1 antibody [EPR16589]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain, hippocampus)
Antigen retrieval step
None
Permeabilization
No
Specification
Brain, hippocampus
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: .3% · Temperature: RT°C
Fixative
Paraformaldehyde
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Collin Gagne

Verified customer

Submitted Jun 21 2019

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Iba1 antibody [EPR16589]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Hippocampus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Citrate buffer, pH 6.0
Permeabilization
Yes - 0.1% Triton in PBS
Specification
Hippocampus
Blocking step
1% BSA + 5% Serum + 0.3 M Glycine as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Sergi Bayod

Verified customer

Submitted Nov 19 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Iba1 antibody [EPR16589]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Epitope retrieval 2 solution (pH=9)
Permeabilization
No
Specification
Brain
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Zohar Gavish

Verified customer

Submitted Sep 26 2018

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