Recombinant Anti-Iba1 antibody [EPR6136(2)] (ab178680)
![Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody [EPR6136(2)] (ab178680)](https://www.abcam.com/ps/products/178/ab178680/Images/ab178680-12-anti-iba1-antibody-epr6136-2-immunocytochemistry-jurkat-human.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody [EPR6136(2)] (ab178680)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6136(2)] to Iba1
- Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Iba1 antibody [EPR6136(2)]
See all Iba1 primary antibodies -
Description
Rabbit monoclonal [EPR6136(2)] to Iba1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: THP-1 cell lysate. Flow Cyt (Intra): K-562 cells. ICC/IF: Jurkat cells. IHC-P: Human kidney, Human tonsil, and Human cerebrum tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6136(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab178680 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| ICC/IF |
1/100.
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| IHC-P |
1/16000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For unpurified use at 1/250 - 1/500. |
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| WB |
1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 16 kDa).
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| Notes |
|---|
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF
1/100. |
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IHC-P
1/16000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For unpurified use at 1/250 - 1/500. |
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WB
1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 16 kDa). |
Target
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Function
Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. -
Tissue specificity
Detected in T-lymphocytes and peripheral blood mononuclear cells. -
Sequence similarities
Contains 2 EF-hand domains. -
Post-translational
modificationsPhosphorylated on serine residues. -
Cellular localization
Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis. - Information by UniProt
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Database links
- Entrez Gene: 199 Human
- Omim: 601833 Human
- SwissProt: P55008 Human
- Unigene: 76364 Human
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Alternative names
- AIF 1 antibody
- AIF-1 antibody
- Aif1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody [EPR6136(2)] (ab178680)Immunocytochemistry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Iba1 with purified ab178680 at 1:100 dilution (9.7 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (2.5 µg/ml) (ab195889) (red). Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as a nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Western blot - Anti-Iba1 antibody [EPR6136(2)] (ab178680)](https://www.abcam.com/ps/products/178/ab178680/Images/ab178680-483924-anti-iba1-antibody-epr6136-2-westernblot-thp1-human.jpg)
Western blot - Anti-Iba1 antibody [EPR6136(2)] (ab178680)Anti-Iba1 antibody [EPR6136(2)] (ab178680) at 1/1000 dilution (Purified) + THP-1 (Human monocytic leukemia monocyte) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 16 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)](https://www.abcam.com/ps/products/178/ab178680/Images/ab178680-11-anti-iba1-antibody-epr6136-2-immunohistochemistry-kidney-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Iba1 with purified ab178680 at 1:16000 (0.06 µg/ml). Heat mediated antigen retrieval was performed using ab93678 (citrate buffer, pH 6.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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![Flow Cytometry (Intracellular) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)](https://www.abcam.com/ps/products/178/ab178680/Images/ab178680-13-anti-iba1-antibody-epr6136-2-flowcytometry-k562-human.jpg)
Flow Cytometry (Intracellular) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labelling Iba1 with purified ab178680 at 1/100 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150081) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (blue). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)](https://www.abcam.com/ps/products/178/ab178680/Images/ab178680-10-anti-iba1-antibody-epr6136-2-immunohistochemistry-tonsil-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling Iba1 with purified ab178680 at 1:16000 (0.06 µg/ml). Heat mediated antigen retrieval was performed using ab93678 (citrate buffer, pH 6.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)](https://www.abcam.com/ps/products/178/ab178680/Images/ab178680-9-anti-iba1-antibody-epr6136-2-immunohistochemistry-cerebrum-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Iba1 with purified ab178680 at 1:16000 (0.06 µg/ml). Heat mediated antigen retrieval was performed using ab93678 (citrate buffer, pH 6.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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![Anti-Iba1 antibody [EPR6136(2)] (ab178680)](https://www.abcam.com/ps/products/178/ab178680/Images/ab178680-8-benefits-of-recombinant-antibodies.png)
Anti-Iba1 antibody [EPR6136(2)] (ab178680)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (21)
ab178680 has been referenced in 21 publications.
- Rey F et al. Mitochondrial Metabolism as Target of the Neuroprotective Role of Erythropoietin in Parkinson's Disease. Antioxidants (Basel) 10:N/A (2021). PubMed: 33467745
- Wassink G et al. Non-additive effects of adjunct erythropoietin therapy with therapeutic hypothermia after global cerebral ischaemia in near-term fetal sheep. J Physiol 598:999-1015 (2020). PubMed: 31912503
- Luo R et al. Activation of PPARA-mediated autophagy reduces Alzheimer disease-like pathology and cognitive decline in a murine model. Autophagy 16:52-69 (2020). PubMed: 30898012
- Camarillo-López RH et al. Tert-butyl-(4-hydroxy-3-((3-(2-methylpiperidin-yl)propyl)carbamoyl)phenyl)carbamate Has Moderated Protective Activity in Astrocytes Stimulated with Amyloid Beta 1-42 and in a Scopolamine Model. Molecules 25:N/A (2020). PubMed: 33137907
- Cho KHT et al. Effects of delayed intraventricular TLR7 agonist administration on long-term neurological outcome following asphyxia in the preterm fetal sheep. Sci Rep 10:6904 (2020). PubMed: 32327682