Recombinant
RabMAb

Recombinant Anti-Iba1 antibody [EPR6136(2)] (ab178680)

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Overview

  • Product name

    Anti-Iba1 antibody [EPR6136(2)]
    See all Iba1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6136(2)] to Iba1

  • Host species

    Rabbit

  • Specificity

    In our hands, ab178680 detects a band at the correct molecular weight, 17 kDa, in human tissue and cell lines in WB. However it did not detect Iba1 in rat or mouse samples. Abcam recommends blocking in 3% milk for this product. Blocking in BSA gives high background.

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Iba1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P55008

  • Positive control

    • IHC-P: Human glioma, hippocampal and kidney tissues; ICC/IF: K562 cells; FC: K562 cells; WB: Human fetal spleen lysate.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab178680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 16 kDa).

Abcam recommends blocking in 3% milk for this product. Blocking in BSA gives high background.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

    • Tissue specificity

      Detected in T-lymphocytes and peripheral blood mononuclear cells.

    • Sequence similarities

      Contains 2 EF-hand domains.

    • Post-translational
      modifications

      Phosphorylated on serine residues.

    • Cellular localization

      Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.
    • Information by UniProt

    • Database links

      • Alternative names

        • AIF 1 antibody
        • AIF-1 antibody
        • Aif1 antibody
        • AIF1 protein antibody
        • AIF1_HUMAN antibody
        • Allograft inflammatory factor 1 antibody
        • Allograft inflammatory factor 1 splice variant G antibody
        • allograft inflammatory factor-1 splice variant Hara-1 antibody
        • balloon angioplasty responsive transcription antibody
        • BART 1 antibody
        • G1 antibody
        • G1 putative splice variant of allograft inflamatory factor 1 antibody
        • IBA 1 antibody
        • IBA1 antibody
        • interferon gamma responsive transcript antibody
        • Interferon responsive transcript 1 antibody
        • interferon responsive transcript factor 1 antibody
        • Ionized calcium binding adapter molecule 1 antibody
        • Ionized calcium-binding adapter molecule 1 antibody
        • ionized calcium-binding adapter molecule antibody
        • IRT 1 antibody
        • IRT1 antibody
        • Microglia response factor antibody
        • MRF1 antibody
        • Protein g1 antibody
        see all

      Images

      • Western blot - Anti-Iba1 antibody [EPR6136(2)] (ab178680)
        Western blot - Anti-Iba1 antibody [EPR6136(2)] (ab178680)
        Anti-Iba1 antibody [EPR6136(2)] (ab178680) at 1/1000 dilution + Human fetal spleen lysate at 15 µg

        Secondary
        Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

        Predicted band size: 16 kDa
        Observed band size: 17 kDa
        why is the actual band size different from the predicted?



        Exposure time: 180 seconds.

        Blocking/Diluting buffer and concentration: 5% NFDM/TBST

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)

        IHC image of Iba1 staining in human normal hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab178680, 1/500 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

         

        For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)

        Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling Iba1 with ab178680 at 1/250 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)

        Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Iba1 with ab178680 at 1/250 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody [EPR6136(2)] (ab178680)
        Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody [EPR6136(2)] (ab178680)

        Immunofluorescent analysis of K562 cells labeling Iba1 with ab178680 (green). DAPI nuclear staining (blue).

      • Flow Cytometry - Anti-Iba1 antibody [EPR6136(2)] (ab178680)
        Flow Cytometry - Anti-Iba1 antibody [EPR6136(2)] (ab178680)

        Flow cytometric analysis of permeabilized K562 cells labeling Iba1 with ab178680 at 1/100 dilution (red) or a rabbit IgG (negative) (green).

      References

      This product has been referenced in:

      • Fioravante M  et al. Hypothalamic expression of the atypical chemokine receptor ACKR2 is involved in the systemic regulation of glucose tolerance. Biochim Biophys Acta Mol Basis Dis 1865:1126-1137 (2019). Read more (PubMed: 30738810) »
      • Cho KHT  et al. Protective effects of delayed intraventricular TLR7 agonist administration on cerebral white and gray matter following asphyxia in the preterm fetal sheep. Sci Rep 9:9562 (2019). Read more (PubMed: 31267031) »
      See all 11 Publications for this product

      Publishing research using ab178680? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      Immunohistochemistry (Frozen sections) abreview for Anti-Iba1 antibody [EPR6136(2)]

       Inconclusive
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
      Sample
      Mouse Tissue sections (brain)
      Specification
      brain
      Permeabilization
      Yes - PBS- 0.25%TritonX
      Fixative
      Paraformaldehyde
      Read More

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      Verified customer

      Submitted Dec 31 2014

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