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    iba1-antibody-epr61362-ab178680.pdf

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RecombinantRabMAb

Recombinant Anti-Iba1 antibody [EPR6136(2)] (ab178680)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (21)

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Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody [EPR6136(2)] (ab178680)
  • Western blot - Anti-Iba1 antibody [EPR6136(2)] (ab178680)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)
  • Flow Cytometry (Intracellular) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)
  • Anti-Iba1 antibody [EPR6136(2)] (ab178680)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6136(2)] to Iba1
  • Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 568 Alexa Fluor® 647 Carrier Free PE

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Protein
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Recombinant Human Iba1 protein (ab105593)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Primary
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Anti-Iba1 antibody [EPR16589] - BSA and Azide free (ab221790)

View more associated products

Overview

  • Product name

    Anti-Iba1 antibody [EPR6136(2)]
    See all Iba1 primary antibodies
  • Description

    Rabbit monoclonal [EPR6136(2)] to Iba1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: THP-1 cell lysate. Flow Cyt (Intra): K-562 cells. ICC/IF: Jurkat cells. IHC-P: Human kidney, Human tonsil, and Human cerebrum tissues.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6136(2)
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Other
    • Immunology
    • Immune Interventions
    • Transplantation
    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Microglia marker
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Iba1 antibody [EPR6136(2)] (ab195031)
    • Alexa Fluor® 647 Anti-Iba1 antibody [EPR6136(2)] (ab195032)
    • PE Anti-Iba1 antibody [EPR6136(2)] (ab209942)
    • Alexa Fluor® 568 Anti-Iba1 antibody [EPR6136(2)] (ab221003)
    • Anti-Iba1 antibody [EPR6136(2)] - BSA and Azide free (ab221933)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Iba1 protein (ab105593)
  • Related Products

    • Recombinant Human Iba1 protein (ab105593)
    • Recombinant Human Iba1 protein (ab173043)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab178680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
1/100.
IHC-P
1/16000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/250 - 1/500.

WB
1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 16 kDa).
Notes
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
1/100.
IHC-P
1/16000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/250 - 1/500.

WB
1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 16 kDa).

Target

  • Function

    Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
  • Tissue specificity

    Detected in T-lymphocytes and peripheral blood mononuclear cells.
  • Sequence similarities

    Contains 2 EF-hand domains.
  • Post-translational
    modifications

    Phosphorylated on serine residues.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.
  • Target information above from: UniProt accession P55008 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 199 Human
    • Omim: 601833 Human
    • SwissProt: P55008 Human
    • Unigene: 76364 Human
    • Alternative names

      • AIF 1 antibody
      • AIF-1 antibody
      • Aif1 antibody
      • AIF1 protein antibody
      • AIF1_HUMAN antibody
      • Allograft inflammatory factor 1 antibody
      • Allograft inflammatory factor 1 splice variant G antibody
      • allograft inflammatory factor-1 splice variant Hara-1 antibody
      • balloon angioplasty responsive transcription antibody
      • BART 1 antibody
      • G1 antibody
      • G1 putative splice variant of allograft inflamatory factor 1 antibody
      • IBA 1 antibody
      • IBA1 antibody
      • interferon gamma responsive transcript antibody
      • Interferon responsive transcript 1 antibody
      • interferon responsive transcript factor 1 antibody
      • Ionized calcium binding adapter molecule 1 antibody
      • Ionized calcium-binding adapter molecule 1 antibody
      • ionized calcium-binding adapter molecule antibody
      • IRT 1 antibody
      • IRT1 antibody
      • Microglia response factor antibody
      • MRF1 antibody
      • Protein g1 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody [EPR6136(2)] (ab178680)
      Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody [EPR6136(2)] (ab178680)
      Immunocytochemistry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Iba1 with purified ab178680 at 1:100 dilution (9.7 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (2.5 µg/ml) (ab195889) (red). Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as a nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Western blot - Anti-Iba1 antibody [EPR6136(2)] (ab178680)
      Western blot - Anti-Iba1 antibody [EPR6136(2)] (ab178680)
      Anti-Iba1 antibody [EPR6136(2)] (ab178680) at 1/1000 dilution (Purified) + THP-1 (Human monocytic leukemia monocyte) whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 16 kDa
      Observed band size: 17 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Iba1 with purified ab178680 at 1:16000 (0.06 µg/ml). Heat mediated antigen retrieval was performed using ab93678 (citrate buffer, pH 6.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    • Flow Cytometry (Intracellular) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)
      Flow Cytometry (Intracellular) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)
      Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labelling Iba1 with purified ab178680 at 1/100 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150081) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (blue).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling Iba1 with purified ab178680 at 1:16000 (0.06 µg/ml). Heat mediated antigen retrieval was performed using ab93678 (citrate buffer, pH 6.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Iba1 with purified ab178680 at 1:16000 (0.06 µg/ml). Heat mediated antigen retrieval was performed using ab93678 (citrate buffer, pH 6.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    • Anti-Iba1 antibody [EPR6136(2)] (ab178680)
      Anti-Iba1 antibody [EPR6136(2)] (ab178680)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (21)

    Publishing research using ab178680? Please let us know so that we can cite the reference in this datasheet.

    ab178680 has been referenced in 21 publications.

    • Rey F  et al. Mitochondrial Metabolism as Target of the Neuroprotective Role of Erythropoietin in Parkinson's Disease. Antioxidants (Basel) 10:N/A (2021). PubMed: 33467745
    • Wassink G  et al. Non-additive effects of adjunct erythropoietin therapy with therapeutic hypothermia after global cerebral ischaemia in near-term fetal sheep. J Physiol 598:999-1015 (2020). PubMed: 31912503
    • Luo R  et al. Activation of PPARA-mediated autophagy reduces Alzheimer disease-like pathology and cognitive decline in a murine model. Autophagy 16:52-69 (2020). PubMed: 30898012
    • Camarillo-López RH  et al. Tert-butyl-(4-hydroxy-3-((3-(2-methylpiperidin-yl)propyl)carbamoyl)phenyl)carbamate Has Moderated Protective Activity in Astrocytes Stimulated with Amyloid Beta 1-42 and in a Scopolamine Model. Molecules 25:N/A (2020). PubMed: 33137907
    • Cho KHT  et al. Effects of delayed intraventricular TLR7 agonist administration on long-term neurological outcome following asphyxia in the preterm fetal sheep. Sci Rep 10:6904 (2020). PubMed: 32327682
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Frozen sections) abreview for Anti-Iba1 antibody [EPR6136(2)]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Sample
    Mouse Tissue sections (brain)
    Specification
    brain
    Permeabilization
    Yes - PBS- 0.25%TritonX
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 31 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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