Product nameIbotenic acid, excitotoxic agonist
Biological descriptionNeuroexcitatory amino acid originally isolated from Amanita species. Neurotoxin often used to model cognitive dysfunctions. NMDA and metabotropic receptor agonist.
Also available in simple stock solutions (ab146670) - add 1 ml of water to get an exact, ready-to-use concentration.
Chemical name2-Amino-2-(3-hydroxyisoxazol-5-yl)acetic acid
Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in water to 10 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
- Receptors & Transporters
- Broad spectrum / non-selective
Representative examples of NeuN immunohistochemistry of a complete auditory cortex lesion or sham-operated control section. Rats were anesthetized with halothane and placed in a Kopf stereotaxic apparatus. The skin overlying the skull was disinfected, an incision made, and small burr holes drilled through the skull bone to allow penetration of the injector (Hamilton 1 µl syringe). Bilateral excitotoxic lesions (two per side) were produced by injections of 0.25 µl ibotenic acid (10 µg/µl in 0.1 M sodium phosphate buffer, pH: 7.4; ab120041). The rate of infusion was 0.05 µl/min. The injector was lowered in the brain and left in place 3 min before and 5 min after each injection.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab120041 has been referenced in 11 publications.
- Guirado R et al. Evidence for Competition for Target Innervation in the Medial Prefrontal Cortex. Cereb Cortex 26:1287-94 (2016). PubMed: 26637448
- Murray JE et al. Basolateral and central amygdala differentially recruit and maintain dorsolateral striatum-dependent cocaine-seeking habits. Nat Commun 6:10088 (2015). PubMed: 26657320
- Poliwoda A et al. Determination of muscimol and ibotenic acid in mushrooms of Amanitaceae by capillary electrophoresis. Electrophoresis 35:2593-9 (2014). PubMed: 24981810
- Pelloux Y et al. The subthalamic nucleus keeps you high on emotion: behavioral consequences of its inactivation. Front Behav Neurosci 8:414 (2014). PubMed: 25538581
- Masini CV et al. Auditory cortex lesions do not disrupt habituation of HPA axis responses to repeated noise stress. Brain Res 1443:18-26 (2012). PubMed: 22290179