- References (11)
Product nameIbotenic acid, excitotoxic agonist
Biological descriptionNeuroexcitatory amino acid originally isolated from Amanita species. Neurotoxin often used to model cognitive dysfunctions. NMDA and metabotropic receptor agonist.
Also available in simple stock solutions (ab146670) - add 1 ml of water to get an exact, ready-to-use concentration.
Chemical name2-Amino-2-(3-hydroxyisoxazol-5-yl)acetic acid
Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in water to 10 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
- Receptors & Transporters
- Broad spectrum / non-selective
Representative examples of NeuN immunohistochemistry of a complete auditory cortex lesion or sham-operated control section. Rats were anesthetized with halothane and placed in a Kopf stereotaxic apparatus. The skin overlying the skull was disinfected, an incision made, and small burr holes drilled through the skull bone to allow penetration of the injector (Hamilton 1 µl syringe). Bilateral excitotoxic lesions (two per side) were produced by injections of 0.25 µl ibotenic acid (10 µg/µl in 0.1 M sodium phosphate buffer, pH: 7.4; ab120041). The rate of infusion was 0.05 µl/min. The injector was lowered in the brain and left in place 3 min before and 5 min after each injection.
This product has been referenced in:
- Eijkenboom M et al. Modelling cognitive dysfunctions with bilateral injections of ibotenic acid into the rat entorhinal cortex. Neuroscience 101:27-39 (2000). Read more (PubMed: 11068134) »
- Inglis WL & Semba K Discriminable excitotoxic effects of ibotenic acid, AMPA, NMDA and quinolinic acid in the rat laterodorsal tegmental nucleus. Brain Res 755:17-27 (1997). Read more (PubMed: 9163537) »