Key features and details
- Rabbit polyclonal to ICAD
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ICAD antibody
See all ICAD primary antibodies
DescriptionRabbit polyclonal to ICAD
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rhesus monkey
Recombinant fragment within Human ICAD (internal sequence). The exact sequence is proprietary.
Database link: O00273
- WB: HEK-293T and Jurkat whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1% BSA, 20% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 37 kDa.|
FunctionInhibitor of the caspase-activated DNase (DFF40).
Sequence similaritiesContains 1 CIDE-N domain.
modificationsCaspase-3 cleaves DFF45 at 2 sites to generate an active factor.
Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- A330085O09Rik antibody
- Caspase activated deoxyribonuclease inhibitor short form antibody
- DFF 1 antibody
All lanes : Anti-ICAD antibody (ab227137) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell ysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell ysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 37 kDa
10% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227137 has not yet been referenced specifically in any publications.