Anti-ICAD antibody (ab264202)
Overview
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Product name
Anti-ICAD antibody
See all ICAD primary antibodies -
Description
Rabbit polyclonal to ICAD -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human ICAD aa 271-331. The exact sequence is proprietary.
Database link: O00273 -
Positive control
- IHC-P: Human ovarian cancer tissue; WB: HeLa and HEK-293T whole cell lysates; IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab264202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-5 µg/mg of lysate. | |
| IHC-P | 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | 1/2000 - 1/10000. |
Target
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Function
Inhibitor of the caspase-activated DNase (DFF40). -
Sequence similarities
Contains 1 CIDE-N domain. -
Post-translational
modificationsCaspase-3 cleaves DFF45 at 2 sites to generate an active factor.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1676 Human
- Omim: 601882 Human
- SwissProt: O00273 Human
- Unigene: 484782 Human
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Alternative names
- A330085O09Rik antibody
- Caspase activated deoxyribonuclease inhibitor short form antibody
- DFF 1 antibody
see all
Images
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Western blot - Anti-ICAD antibody (ab264202)All lanes : Anti-ICAD antibody (ab264202) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µgDetected with chemilimuniscence with exposure time of 30 seconds.
4-20% SDS-PAGE.
Secondary: Goat anti-Rabbit Light Chain HRP Conjugate.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAD antibody (ab264202)Immunohistochemical analysis of formalin/PFA-fixed, paraffin-embedded human ovarian tissue sections labeling ICAD with ab264202 at 1/200 dilution. Detection: DAB.
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Immunoprecipitation - Anti-ICAD antibody (ab264202)ICAD was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264202 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264202 at 1 µg/ml.
Lane 1: ab264202 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 1 sec.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References
ab264202 has not yet been referenced specifically in any publications.
