Product nameAnti-ICAD antibody
See all ICAD primary antibodies
DescriptionRabbit polyclonal to ICAD
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human ICAD aa 271-331. The exact sequence is proprietary.
Database link: O00273
- IHC-P: Human ovarian cancer tissue; WB: HeLa and HEK-293T whole cell lysates; IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264202 in the following tested applications.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|WB||1/2000 - 1/10000.|
FunctionInhibitor of the caspase-activated DNase (DFF40).
Sequence similaritiesContains 1 CIDE-N domain.
modificationsCaspase-3 cleaves DFF45 at 2 sites to generate an active factor.
Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- A330085O09Rik antibody
- Caspase activated deoxyribonuclease inhibitor short form antibody
- DFF 1 antibody
All lanes : Anti-ICAD antibody (ab264202) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Detected with chemilimuniscence with exposure time of 30 seconds.
Secondary: Goat anti-Rabbit Light Chain HRP Conjugate.
Immunohistochemical analysis of formalin/PFA-fixed, paraffin-embedded human ovarian tissue sections labeling ICAD with ab264202 at 1/200 dilution. Detection: DAB.
ICAD was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264202 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264202 at 1 µg/ml.
Lane 1: ab264202 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 1 sec.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264202 has not yet been referenced specifically in any publications.