Recombinant
RabMAb

Recombinant Anti-ICAD antibody [EPR4221] (ab133250)

Overview

  • Product name

    Anti-ICAD antibody [EPR4221]
    See all ICAD primary antibodies
  • Description

    Rabbit monoclonal [EPR4221] to ICAD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ICAD aa 300-400 (C terminal). The exact sequence is proprietary.
    Database link: O00273

  • Positive control

    • Jurkat; U937; A431 and HeLa cell lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 37 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Inhibitor of the caspase-activated DNase (DFF40).
    • Sequence similarities

      Contains 1 CIDE-N domain.
    • Post-translational
      modifications

      Caspase-3 cleaves DFF45 at 2 sites to generate an active factor.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • A330085O09Rik antibody
      • Caspase activated deoxyribonuclease inhibitor short form antibody
      • DFF 1 antibody
      • DFF 45 antibody
      • DFF alpha antibody
      • DFF-45 antibody
      • DFF1 antibody
      • DFF35 antibody
      • DFF45 antibody
      • DFFA antibody
      • Dffa DNA fragmentation factor, alpha subunit antibody
      • DFFA_HUMAN antibody
      • DNA fragmentation factor 45 kDa subunit antibody
      • DNA Fragmentation Factor Alpha Subunit antibody
      • DNA fragmentation factor subunit alpha antibody
      • DNA fragmentation factor, 45 kD, alpha subunit antibody
      • DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1 antibody
      • DNA fragmentation factor, 45kDa, alpha polypeptide antibody
      • DNA fragmentation factor, alpha subunit antibody
      • DNAation factor 45 kDa subunit antibody
      • H13 antibody
      • ICAD antibody
      • ICAD L antibody
      • ICAD S antibody
      • Inhibitor of CAD antibody
      • Inhibitor of Caspase Activated DNase antibody
      • MGC143066 antibody
      • OTTHUMP00000001903 antibody
      • OTTHUMP00000001904 antibody
      • RP23 121D17.3 antibody
      see all

    Images

    • All lanes : Anti-ICAD antibody [EPR4221] (ab133250) at 1/10000 dilution

      Lane 1 : Jurkat cell lysates
      Lane 2 : U937 cell lysates
      Lane 3 : A431 cell lysates
      Lane 4 : HeLa cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 37 kDa

    References

    ab133250 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133250.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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