Recombinant Anti-ICAD antibody [EPR4222(2)] (ab108521)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4222(2)] to ICAD
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ICAD antibody [EPR4222(2)]
See all ICAD primary antibodies -
Description
Rabbit monoclonal [EPR4222(2)] to ICAD -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human ICAD aa 200-300. The exact sequence is proprietary.
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Positive control
- HepG2, U937, A431, and A549 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4222(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab108521 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 37 kDa. |
Target
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Function
Inhibitor of the caspase-activated DNase (DFF40). -
Sequence similarities
Contains 1 CIDE-N domain. -
Post-translational
modificationsCaspase-3 cleaves DFF45 at 2 sites to generate an active factor.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1676 Human
- Entrez Gene: 13347 Mouse
- Entrez Gene: 114214 Rat
- Omim: 601882 Human
- SwissProt: O00273 Human
- SwissProt: O54786 Mouse
- Unigene: 484782 Human
- Unigene: 41433 Mouse
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Alternative names
- A330085O09Rik antibody
- Caspase activated deoxyribonuclease inhibitor short form antibody
- DFF 1 antibody
see all
Images
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![Western blot - Anti-ICAD antibody [EPR4222(2)] (ab108521)](https://www.abcam.com/ps/products/108/ab108521/Images/ab108521-121237-anti-icad-antibody-epr42222-western-blot.jpg)
Western blot - Anti-ICAD antibody [EPR4222(2)] (ab108521)All lanes : Anti-ICAD antibody [EPR4222(2)] (ab108521) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : U937 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 37 kDa -
![Anti-ICAD antibody [EPR4222(2)] (ab108521)](https://www.abcam.com/ps/products/108/ab108521/Images/ab108521-2-benefits-of-recombinant-antibodies.png)
Anti-ICAD antibody [EPR4222(2)] (ab108521)
Datasheets and documents
References (1)
ab108521 has been referenced in 1 publication.
- Wang FZ et al. Molecular mechanisms underlying the a-tomatine-directed apoptosis in human malignant glioblastoma cell lines A172 and U-118 MG. Exp Ther Med 14:6183-6192 (2017). PubMed: 29250143
