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    icad-antibody-epr42222-ab108521.pdf

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Cell Biology Apoptosis Nucleus DNAses
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RecombinantRabMAb

Recombinant Anti-ICAD antibody [EPR4222(2)] (ab108521)

  • Datasheet
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Western blot - Anti-ICAD antibody [EPR4222(2)] (ab108521)
  • Anti-ICAD antibody [EPR4222(2)] (ab108521)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4222(2)] to ICAD
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-ICAD antibody [EPR4222(2)]
    See all ICAD primary antibodies
  • Description

    Rabbit monoclonal [EPR4222(2)] to ICAD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, U937, A431, and A549 cell lysates
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR4222(2)
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Nucleus
    • DNAses
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Apoptosis
    • Nuclear
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Nucleus
    • DNAses
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Alternative Versions

    • Anti-ICAD antibody [EPR4222(2)] - BSA and Azide free (ab247681)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • U937 cytoplasmic extract lysate (ab14904)
  • Recombinant Protein

    • Recombinant Human ICAD protein (ab173052)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 37 kDa.
Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 37 kDa.
Application notes
Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

Target

  • Function

    Inhibitor of the caspase-activated DNase (DFF40).
  • Sequence similarities

    Contains 1 CIDE-N domain.
  • Post-translational
    modifications

    Caspase-3 cleaves DFF45 at 2 sites to generate an active factor.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession O00273 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1676 Human
    • Entrez Gene: 13347 Mouse
    • Entrez Gene: 114214 Rat
    • Omim: 601882 Human
    • SwissProt: O00273 Human
    • SwissProt: O54786 Mouse
    • Unigene: 484782 Human
    • Unigene: 41433 Mouse
    • Alternative names

      • A330085O09Rik antibody
      • Caspase activated deoxyribonuclease inhibitor short form antibody
      • DFF 1 antibody
      • DFF 45 antibody
      • DFF alpha antibody
      • DFF-45 antibody
      • DFF1 antibody
      • DFF35 antibody
      • DFF45 antibody
      • DFFA antibody
      • Dffa DNA fragmentation factor, alpha subunit antibody
      • DFFA_HUMAN antibody
      • DNA fragmentation factor 45 kDa subunit antibody
      • DNA Fragmentation Factor Alpha Subunit antibody
      • DNA fragmentation factor subunit alpha antibody
      • DNA fragmentation factor, 45 kD, alpha subunit antibody
      • DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1 antibody
      • DNA fragmentation factor, 45kDa, alpha polypeptide antibody
      • DNA fragmentation factor, alpha subunit antibody
      • DNAation factor 45 kDa subunit antibody
      • H13 antibody
      • ICAD antibody
      • ICAD L antibody
      • ICAD S antibody
      • Inhibitor of CAD antibody
      • Inhibitor of Caspase Activated DNase antibody
      • MGC143066 antibody
      • OTTHUMP00000001903 antibody
      • OTTHUMP00000001904 antibody
      • RP23 121D17.3 antibody
      see all

    Images

    • Western blot - Anti-ICAD antibody [EPR4222(2)] (ab108521)
      Western blot - Anti-ICAD antibody [EPR4222(2)] (ab108521)
      All lanes : Anti-ICAD antibody [EPR4222(2)] (ab108521) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : U937 cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 37 kDa

    • Anti-ICAD antibody [EPR4222(2)] (ab108521)
      Anti-ICAD antibody [EPR4222(2)] (ab108521)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (1)

    Publishing research using ab108521? Please let us know so that we can cite the reference in this datasheet.

    ab108521 has been referenced in 1 publication.

    • Wang FZ  et al. Molecular mechanisms underlying the a-tomatine-directed apoptosis in human malignant glioblastoma cell lines A172 and U-118 MG. Exp Ther Med 14:6183-6192 (2017). PubMed: 29250143

    Customer reviews and Q&As

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