Recombinant Anti-ICAD antibody [EPR4222(2)] (ab108521)
![Western blot - Anti-ICAD antibody [EPR4222(2)] (ab108521)](https://www.abcam.com/ps/products/108/ab108521/Images/ab108521-121237-anti-icad-antibody-epr42222-western-blot.jpg "Western blot - Anti-ICAD antibody [EPR4222(2)] (ab108521)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4222(2)] to ICAD
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ICAD antibody [EPR4222(2)]
See all ICAD primary antibodies -
Description
Rabbit monoclonal [EPR4222(2)] to ICAD -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2, U937, A431, and A549 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4222(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108521 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 37 kDa.
|
| Notes |
|---|
|
WB
1/10000 - 1/50000. Predicted molecular weight: 37 kDa. |
Application notes
Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
Target
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Function
Inhibitor of the caspase-activated DNase (DFF40). -
Sequence similarities
Contains 1 CIDE-N domain. -
Post-translational
modificationsCaspase-3 cleaves DFF45 at 2 sites to generate an active factor.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1676 Human
- Entrez Gene: 13347 Mouse
- Entrez Gene: 114214 Rat
- Omim: 601882 Human
- SwissProt: O00273 Human
- SwissProt: O54786 Mouse
- Unigene: 484782 Human
- Unigene: 41433 Mouse
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Alternative names
- A330085O09Rik antibody
- Caspase activated deoxyribonuclease inhibitor short form antibody
- DFF 1 antibody
see all
Images
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Western blot - Anti-ICAD antibody [EPR4222(2)] (ab108521)All lanes : Anti-ICAD antibody [EPR4222(2)] (ab108521) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : U937 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 37 kDa -
![Anti-ICAD antibody [EPR4222(2)] (ab108521)](https://www.abcam.com/ps/products/108/ab108521/Images/ab108521-2-benefits-of-recombinant-antibodies.png)
Anti-ICAD antibody [EPR4222(2)] (ab108521)
Datasheets and documents
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Datasheet download
References (1)
ab108521 has been referenced in 1 publication.
- Wang FZ et al. Molecular mechanisms underlying the a-tomatine-directed apoptosis in human malignant glioblastoma cell lines A172 and U-118 MG. Exp Ther Med 14:6183-6192 (2017). PubMed: 29250143
