Product nameICAD overexpression 293T lysate (whole cell)
ab94130 is a 293T cell transfected lysate in which Human ICAD has been transiently over-expressed using a pCMV-ICAD plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundFunction: Inhibitor of the caspase-activated DNase (DFF40). Similarity: Contains 1 CIDE-N domain. PTM: Caspase-3 cleaves DFF45 at 2 sites to generate an active factor. Phosphorylated upon DNA damage, probably by ATM or ATR.
Our Abpromise guarantee covers the use of ab94130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94130 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-ICAD antibody (ab90563) at 1/500 dilution
Lane 1 :
ICAD overexpression 293T lysate (whole cell) (ab94130)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94130 has not yet been referenced specifically in any publications.