Product nameAnti-ICAM1 antibody [1A29]
See all ICAM1 primary antibodies
DescriptionMouse monoclonal [1A29] to ICAM1
SpecificityRecognises a 90kD adhesion molecule belonging to the immunoglobulin superfamily.
Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Fr, IP, WB, Functional Studies, Flow Cytmore details
Species reactivityReacts with: Rat
Tissue/ cell preparation (Rat) -Ax cells (a HEV derived cell line).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: None, No stabilisers, Low endotoxin levels -<0.01EU/µg
Constituents: PBS, pH 7.4
Concentration information loading...
PurityProtein G purified
Purification notesab33894 was purified from tissue culture supernatant
Our Abpromise guarantee covers the use of ab33894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 58 kDa.|
|Functional Studies||Use at an assay dependent concentration. ab33894 functionally inhibits homotypic aggregation of PHA blasts.|
|Flow Cyt||1/25 - 1/50.
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
Sequence similaritiesBelongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
modificationsMonoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
- Information by UniProt
- Antigen identified by monoclonal antibody BB2 antibody
- BB 2 antibody
- BB2 antibody
This product has been referenced in:
- Williams BA et al. Impact of arteriogenesis in plastic surgery: choke vessel growth proceeds via arteriogenic mechanisms in the rat dorsal island skin flap. Microcirculation 16:235-50 (2009). WB ; Rat . Read more (PubMed: 19225983) »
- Conde-Vancells J et al. Characterization and comprehensive proteome profiling of exosomes secreted by hepatocytes. J Proteome Res 7:5157-66 (2008). WB ; Rat . Read more (PubMed: 19367702) »