Overview

  • Product name
    Anti-ICAM1 antibody [1A29]
    See all ICAM1 primary antibodies
  • Description
    Mouse monoclonal [1A29] to ICAM1
  • Host species
    Mouse
  • Specificity
    Recognises a 90kD adhesion molecule belonging to the immunoglobulin superfamily.
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IHC-Fr, IP, WB, Functional Studies, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Tissue/ cell preparation (Rat) -Ax cells (a HEV derived cell line).

Properties

Applications

Our Abpromise guarantee covers the use of ab33894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 58 kDa.
Functional Studies Use at an assay dependent concentration. ab33894 functionally inhibits homotypic aggregation of PHA blasts.
Flow Cyt 1/25 - 1/50.

Use 10ul of the suggested working dilution to label 106 cells in 100ul.

 

 

 

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. ICAM family.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen identified by monoclonal antibody BB2 antibody
    • BB 2 antibody
    • BB2 antibody
    • CD 54 antibody
    • CD_antigen=CD54 antibody
    • CD54 antibody
    • Cell surface glycoprotein P3.58 antibody
    • Human rhinovirus receptor antibody
    • ICAM 1 antibody
    • ICAM-1 antibody
    • ICAM1 antibody
    • ICAM1_HUMAN antibody
    • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
    • Intercellular adhesion molecule 1 antibody
    • Major group rhinovirus receptor antibody
    • MALA 2 antibody
    • MALA2 antibody
    • MyD 10 antibody
    • MyD10 antibody
    • P3.58 antibody
    • Surface antigen of activated B cells, BB2 antibody
    see all

References

This product has been referenced in:
  • Williams BA  et al. Impact of arteriogenesis in plastic surgery: choke vessel growth proceeds via arteriogenic mechanisms in the rat dorsal island skin flap. Microcirculation 16:235-50 (2009). WB ; Rat . Read more (PubMed: 19225983) »
  • Conde-Vancells J  et al. Characterization and comprehensive proteome profiling of exosomes secreted by hepatocytes. J Proteome Res 7:5157-66 (2008). WB ; Rat . Read more (PubMed: 19367702) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number . To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

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Answer

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

Reviewing this case, I would like to offer some suggestions to help optimise the results from ab33894.

-It is important to emphasize that there are at least 10 potential N-glucosylation sites along the polypeptide chain of ICAM1. As it is a glycoprotein it contains one or more covalently linked carbohydrates of various types, i.e. from monosaccharides to branched polysaccharides, including glycosylphosphatidylinositol (GPI), glycosaminoglycans (GAG), this modification will affect the MW and could lead to numerous bands at different MW depending on the sample processing.

-In cases where multiple non specific bands are present, the use of a positive control becomes indispensable to help identify the target protein. ICAM1 is highly expressed in lung, placenta and liver tissue.

- Reduce protein load from 30ug to 15ug.

-During sample processing, it is important to disrupt the disulphide bonds. I would therefore recommend increasing the reducing conditions.

-Insufficient blocking conditions could also lead to multiple bands. I would recommend increasing the blocking conditions to Milk 5% during 2 hours at RT.

- Regarding the primary antibody, I would suggest a less concentrated dilution, e.g. 1/250 or 1/500, incubating at 4C overnight.

-Increase the washing conditions. I would suggest performing 4 x 10 minutes washings.

-Run a non primary control to dismiss non specific bands coming from the secondary.

I hope this suggestions help improve the results from the antibody. Please do not hesitate to contact us for further assistance.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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