Overview

  • Product name

  • Description

    Goat polyclonal to ICAM1
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human ICAM1 aa 94-105 (internal sequence). NP_000192.2.
    Sequence:

    SNCPDGQSTAKT


    Database link: P05362

  • Positive control

    • WB: HeLa, Daudi and NIH/3T3 cell lysates.
  • General notes

    The immunizing peptide represents part of the extracellular domain, and it does not overlap any known glycosite.

Properties

Applications

Our Abpromise guarantee covers the use of ab223659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 57.8 kDa).

Target

  • Function

    ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. ICAM family.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications

    Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Antigen identified by monoclonal antibody BB2 antibody
    • BB 2 antibody
    • BB2 antibody
    • CD 54 antibody
    • CD_antigen=CD54 antibody
    • CD54 antibody
    • Cell surface glycoprotein P3.58 antibody
    • Human rhinovirus receptor antibody
    • ICAM 1 antibody
    • ICAM-1 antibody
    • ICAM1 antibody
    • ICAM1_HUMAN antibody
    • intercellular adhesion molecule 1 (CD54) antibody
    • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
    • Intercellular adhesion molecule 1 antibody
    • Major group rhinovirus receptor antibody
    • MALA 2 antibody
    • MALA2 antibody
    • MyD 10 antibody
    • MyD10 antibody
    • P3.58 antibody
    • Surface antigen of activated B cells antibody
    • Surface antigen of activated B cells, BB2 antibody
    see all

Images

  • All lanes : Anti-ICAM1 antibody (ab223659) at 2 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate (in RIPA buffer)
    Lane 2 : Daudi (human Burkitt's lymphoma cell line) cell lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 57.8 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

    The observed molecular weight corresponds to earlier findings in literature (PMID: 19661147).

  • Anti-ICAM1 antibody (ab223659) at 2 µg/ml + NIH/3T3 (mouse embryo fibroblast cell line) cell lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 57.8 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

    The observed molecular weight corresponds to earlier findings in literature (PMID: 19661147).

References

This product has been referenced in:

  • Wang B  et al. miR-577 suppresses cell proliferation and epithelial-mesenchymal transition by regulating the WNT2B mediated Wnt/ß-catenin pathway in non-small cell lung cancer. Mol Med Rep 18:2753-2761 (2018). Read more (PubMed: 30015869) »
See 1 Publication for this product

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