Product nameAnti-ICAM1 antibody (Biotin)
See all ICAM1 primary antibodies
DescriptionRabbit polyclonal to ICAM1 (Biotin)
Tested applicationsSuitable for: Sandwich ELISA, IHC-Fr, IP, WBmore details
Species reactivityReacts with: Mouse, Human, Chimpanzee, Baboon, Cynomolgus monkey, Rhesus monkey
Full length protein (Human).
- Fresh skin biopsy (small fresh skin biopsy incubated for 4 hours with TNF (1 ng/ml), IL-1 (100 u/ml) or LPS (1 ìg/ml) in tissue culture medium at 37oC).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.065% Sodium azide
Constituent: 0.1% BSA
Concentration information loading...
Purification notes0.2 µm filtered
sELISA pair antibody
Our Abpromise guarantee covers the use of ab7815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [MEM-111] to ICAM1 (ab2213).
For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab2213 as Capture.
|IHC-Fr||Use at an assay dependent concentration. Fix with acetone.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
FunctionICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
Sequence similaritiesBelongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
modificationsMonoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
- Information by UniProt
- Antigen identified by monoclonal antibody BB2 antibody
- BB 2 antibody
- BB2 antibody
This product has been referenced in:
- Zhang Q et al. Toll-like receptor 4 contributes to acute kidney injury after cardiopulmonary resuscitation in mice. Mol Med Rep 14:2983-90 (2016). Mouse . Read more (PubMed: 27510583) »
- Rohlena J et al. Endothelial CD81 is a marker of early human atherosclerotic plaques and facilitates monocyte adhesion. Cardiovasc Res 81:187-96 (2009). Electron Microscopy ; Human . Read more (PubMed: 18805782) »