Overview

  • Product name
    Anti-ICAM1 antibody (Biotin)
    See all ICAM1 primary antibodies
  • Description
    Rabbit polyclonal to ICAM1 (Biotin)
  • Host species
    Rabbit
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: Sandwich ELISA, IHC-Fr, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human, Chimpanzee, Baboon, Cynomolgus monkey, Rhesus monkey
  • Immunogen

    Full length protein (Human).

  • Positive control
    • Fresh skin biopsy (small fresh skin biopsy incubated for 4 hours with TNF (1 ng/ml), IL-1 (100 u/ml) or LPS (1 ìg/ml) in tissue culture medium at 37oC).

Properties

Applications

Our Abpromise guarantee covers the use of ab7815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [MEM-111] to ICAM1 (ab2213).

For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab2213 as Capture.

IHC-Fr Use at an assay dependent concentration. Fix with acetone.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

Target

  • Function
    ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. ICAM family.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen identified by monoclonal antibody BB2 antibody
    • BB 2 antibody
    • BB2 antibody
    • CD 54 antibody
    • CD_antigen=CD54 antibody
    • CD54 antibody
    • Cell surface glycoprotein P3.58 antibody
    • Human rhinovirus receptor antibody
    • ICAM 1 antibody
    • ICAM-1 antibody
    • ICAM1 antibody
    • ICAM1_HUMAN antibody
    • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
    • Intercellular adhesion molecule 1 antibody
    • Major group rhinovirus receptor antibody
    • MALA 2 antibody
    • MALA2 antibody
    • MyD 10 antibody
    • MyD10 antibody
    • P3.58 antibody
    • Surface antigen of activated B cells, BB2 antibody
    see all

Images

References

This product has been referenced in:
  • Zhang Q  et al. Toll-like receptor 4 contributes to acute kidney injury after cardiopulmonary resuscitation in mice. Mol Med Rep 14:2983-90 (2016). Mouse . Read more (PubMed: 27510583) »
  • Rohlena J  et al. Endothelial CD81 is a marker of early human atherosclerotic plaques and facilitates monocyte adhesion. Cardiovasc Res 81:187-96 (2009). Electron Microscopy ; Human . Read more (PubMed: 18805782) »
See all 4 Publications for this product

Customer reviews and Q&As

1-10 of 13 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Rhesus monkey Cell (choroidal endothelial cells)
Permeabilization
No
Gating Strategy
size and granularity
Specification
choroidal endothelial cells
Preparation
Cell harvesting/tissue preparation method: cells were detached from tissue culture plates with Accutase
Sample buffer: 2mM EDTA + 0.5% BSA in PBS
Fixation
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 14 2017

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Mouse Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Nov 27 2013

Question
Answer

Apres plusieurs conversations avec notre service commercial j’ai réussi d’ajouter l’anticorps ab7815 non conjugué au catalogue. La référence est ab170497.

Read More

Answer

Thank you for the response.

Any monoclonal antibody is produced by a single immune cell, which has been cloned. These will only recognize a single epitope of the target protein. This epitope typically is 5-10 amino acids in length. Therefore any single monoclonal antibody will only recognize a region within a single domain and not stain both the intracellular and extracellular region of the target protein.

Polyclonal antibodies are created by many different immune cells and will contain multiple epitope recognition sites. Although the amount of antibody specific to a particular epitope will vary from lot-to-lot, it is possible for a polyclonal to therefore contain antibodies which will recognize different domains.

I have reviewed the antibodies which you have listed as well as other polyclonals in our catalog to try and find a product which might be able to recognize the intracellular and extracellular regions.

ab2213Anti-ICAM1 antibody [MEM-111]: This monoclonal antibody was created with an immunogen from a whole tissue / cell preparation (Human). We have not mapped the exact location of the epitope. Therefore the epitope recognized might be intracellular, transmembrane or extracellular.

ab25375Anti-ICAM1 antibody [YN1/1.7.4]: This product is also monoclonal.The details of the immunogen for this antibody are not available. However as a monoclonal, this product will not be able to recognize sites in multiple domains.

ab7815Anti-ICAM1 antibody (Biotin): This product is a rabbit polyclonal and has been raised against the whole protein. As such it is possible that the product will contain antibodies against both extracellular and intracellular regions. We do not test for this type of response and cannot guarantee that the productwill recognizeboth domains. Please be aware that this product is also biotin labeled.

Our other rabbit polyclonal antibodies were each created from recombinant fragments and would also not be able to recognize both extracellular and intracellular domains.

I hope this information is helpful. Please do not hesitate to contact me with further questions.

Read More

Answer

Thank you for contacting us.

ab7815 and ab33451 have been tested with Frozen sections only and we do not have any empirical data to support their performance with paraffin tissues.

I have found the following ICAM1 and Monocyte + macrophage antibodies which have been tested in IHC-P and might be of your interest.

Anti-ICAM1 antibody:

ab2213 ab109361 ab33894. They are not biotin labelled.

Anti-Monocyte + Macrophage antibody


ab51934. React with human.



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for your email.

I have a couple recommendation for your customer when using IHC-Fr.

1.) I strongly suggest to run a positive control. ICAM1 belongs to the homing receptors and to my knowledge should not be expressed in brain. These homing receptor are expressed in site with inflammation to accumulate lymphocytes. If treated skin biopsies are not available, lung or bone marrow might be suitable.

2.) According to the protocol your customer send us, the mouse brain is fixed twice: perfusion fixed and fixed for 4days after. This strong (double-)fixation can lead to confirmation changes in the target protein which renders the antibody unable to recognize the target protein.
I recommend to try various antigen retrieval methods. This has to be tested experimentally.

3.) Your customer is correct, this antibody is labeled with biotin. Without using a secondary antibody there is less amplification of the signal and I therefore suggest to dilute the antibody less and to try 1:50.

I hope these tips can help improve the result. Please do not hesitate to contact me again with any further questions.

Read More

Answer

Thank you and your customer for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from this antibody. I can confirm that ab7815 is covered by our Abpromise guarantee when used in WB.

The details your customer has kindly provided will enable us to investigate this case for your customer and this is also helpful in our records for monitoring of quality.

Reviewing this case, I would like to offer some suggestions to help optimise the results from ab7815:

1.) I suggest use a positive control: Fresh skin biopsy (small fresh skin biopsy incubated for 4 hours with TNF (1 ng/ml), IL-1 (100 u/ml) or LPS (1 ìg/ml) in tissue culture medium at 37oC).

2.) I recommend to use reducing and denaturing conditions: add SDS to the lysis puffer and add beta mercaptho-ethanol to the loading buffer.

3.) Blocking in BSA can significantly increase the signal of some antibodies. Therefore I suggest to use 5% BSA as blocking agent.

4.) Since there is no background I suggest to not use any blocking agent in the antibody solution and to dilute the antibody less. An other customer had good results with a dilution of 1:500.

We are happy to offer this technical support. In the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I hope this information is helpful, thank you and your customer for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

Read More

Question
Answer



I can confirm that we didn't test the antibody (ab106777: Anti-VCAM1) in Rabbit tissue, as soon as we have further information we will update our on-line datasheet accordingly.

Read More

Question
Answer

Thank you for your interest.



The reactivity of this antibody (ab2213) with rabbit samples on Western blot application is probable but it has not been tested yet.



Hope this will be useful for you!

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Loading amount
40 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Alicia Moreno

Verified customer

Submitted Sep 06 2010

1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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