Product nameAnti-ICAM1 antibody [EP1442Y]
See all ICAM1 primary antibodies
DescriptionRabbit monoclonal [EP1442Y] to ICAM1
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human ICAM1 aa 1-100 (N terminal). The exact sequence is proprietary.
(Peptide available as
- WB: Raji cell membrane lysate. IHC-P: Human tonsil tissue. Flow Cyt: Raji cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab53013 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Detects a band of approximately 89 kDa (predicted molecular weight: 58 kDa).Can be blocked with ICAM1 peptide (ab218845).|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||1/100 - 1/250.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
Sequence similaritiesBelongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
modificationsMonoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
- Information by UniProt
- Antigen identified by monoclonal antibody BB2 antibody
- BB 2 antibody
- BB2 antibody
All lanes : Anti-ICAM1 antibody [EP1442Y] (ab53013) at 1/1000 dilution
Lane 1 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 3 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
Lane 4 : THP1 (Human acute monocytic leukemia cell line) + LPS Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
s produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab53013 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling ICAM1 with ab53013 at 1/1000. Heat mediated antigen retrieval was performed using ab93684, Tris/EDTA buffer pH 9. A ready to use HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody. Counterstained with hematoxylin.
Overlay histogram showing Raji cells stained with ab53013 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53013, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Anti-ICAM1 antibody [EP1442Y] (ab53013) at 1/2000 dilution + Raji cell lysate at 10 µg
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 58 kDa
Observed band size: 89 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Giulbudagian M et al. Breaking the Barrier - Potent Anti-Inflammatory Activity following Efficient Topical Delivery of Etanercept using Thermoresponsive Nanogels. Theranostics 8:450-463 (2018). Read more (PubMed: 29290820) »
- Barrera V et al. Neurovascular sequestration in paediatric P. falciparum malaria is visible clinically in the retina. Elife 7:N/A (2018). Read more (PubMed: 29578406) »