Recombinant Anti-ICAM1 antibody [EP1442Y] (ab53013)


  • Product name

    Anti-ICAM1 antibody [EP1442Y]
    See all ICAM1 primary antibodies
  • Description

    Rabbit monoclonal [EP1442Y] to ICAM1
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ICAM1 aa 1-100 (N terminal). The exact sequence is proprietary.
    (Peptide available as ab218845)

  • Positive control

    • WB: Raji cell membrane lysate. IHC-P: Human tonsil tissue. Flow Cyt: Raji cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab53013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 89 kDa (predicted molecular weight: 58 kDa).Can be blocked with ICAM1 peptide (ab218845).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
    • Sequence similarities

      Belongs to the immunoglobulin superfamily. ICAM family.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    • Post-translational

      Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
    • Cellular localization

    • Information by UniProt
    • Database links

    • Alternative names

      • Antigen identified by monoclonal antibody BB2 antibody
      • BB 2 antibody
      • BB2 antibody
      • CD 54 antibody
      • CD_antigen=CD54 antibody
      • CD54 antibody
      • Cell surface glycoprotein P3.58 antibody
      • Human rhinovirus receptor antibody
      • ICAM 1 antibody
      • ICAM-1 antibody
      • ICAM1 antibody
      • ICAM1_HUMAN antibody
      • intercellular adhesion molecule 1 (CD54) antibody
      • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
      • Intercellular adhesion molecule 1 antibody
      • Major group rhinovirus receptor antibody
      • MALA 2 antibody
      • MALA2 antibody
      • MyD 10 antibody
      • MyD10 antibody
      • P3.58 antibody
      • Surface antigen of activated B cells antibody
      • Surface antigen of activated B cells, BB2 antibody
      see all


    • All lanes : Anti-ICAM1 antibody [EP1442Y] (ab53013) at 1/1000 dilution

      Lane 1 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
      Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
      Lane 3 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
      Lane 4 : THP1 (Human acute monocytic leukemia cell line) + LPS Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 58 kDa
      Observed band size: 100 kDa
      why is the actual band size different from the predicted?

      Exposure time: 20 minutes

      s produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab53013 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling ICAM1 with ab53013 at 1/1000. Heat mediated antigen retrieval was performed using ab93684, Tris/EDTA buffer pH 9. A ready to use HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody. Counterstained with hematoxylin.

    • Overlay histogram showing Raji cells stained with ab53013 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53013, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    • Anti-ICAM1 antibody [EP1442Y] (ab53013) at 1/2000 dilution + Raji cell lysate at 10 µg

      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 58 kDa
      Observed band size: 89 kDa why is the actual band size different from the predicted?


    This product has been referenced in:

    • Zhang W  et al. Effect of PA-MSAH preprocessing on the expression of TLR-4-NF-?B pathway and inflammatory factors in the intestinal tract of rats with septic shock. Exp Ther Med 17:2567-2574 (2019). Read more (PubMed: 30906447) »
    • Yin Z  et al. Macrophage-derived exosomal microRNA-501-3p promotes progression of pancreatic ductal adenocarcinoma through the TGFBR3-mediated TGF-ß signaling pathway. J Exp Clin Cancer Res 38:310 (2019). Read more (PubMed: 31307515) »
    See all 29 Publications for this product

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A


    Thank you for contacting us.

    The concentration for lot GR27346-6 is XXXX mg/ml.

    The conc. for lot GR10519-3 is XXXX mg/ml.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Thank you for calling Abcam earlier today.

    I am sorry that you were having issue with ab53013 in IHC. As we discussed on the telephone, I am sending you a vial of ab109361 as a free of charge replacement. Your new order number for tracking purposes only is *.

    If there is anything else I can help you with, or if you experience any issues with the replacement antibody, then please let me know.

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    Thank you for kindly confirming these details as this enables us to closely monitor the quality of our products

    Weare pleased to arrange a replacement product free of charge. I have added now one free unit of ab53013,xx We are currently awaiting the vial to be transferred from the US office.To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should youthe customer stillbe experiencing difficulties, or if you have any further questions, please do not hesitate to let us know

    Thank you for your help and cooperation. Please do not hesitate to contact us if you need anything further.

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    Thank you very muchfor taking time to complete our questionnaire and the additional information. Even though we have not yet tested this antibody in frozen sections, I would expect the new batch to work as the previous one as this is a monoclonal antibody. Indeed it appears that you may have received a faulty vial. I am very sorry for this inconvenience. As said before, we do not have received any negative feedback on this antibody during the last three years. The vial you received might has been damaged during the transport or storage.

    I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compensation. We do have currently the lot GR10519-7 in stock in our US office. Would you like to receive a new vial of this lot?

    Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

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    Thank you for contacting us.

    I am sorry to hear you are experiencing difficulties with one of our products. We have not received any product complaints of this antibody during the past three years and have also no other indication that there might a problem with a batch. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

    Even though in this case it seems to be a straigthforward batch issue, I would nevertheless appreciate if we could gather more details of the experimental procedure. It should not take more than 5 to 10 minutes to the customer. I am attaching therefore our questionnaire which will help to put together somefurther information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results - which I do not expect in this case. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

    I look forward to receiving your reply.

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