Recombinant
Anti-ICAM1 antibody [EPR4776] (ab109361)
- Datasheet
- Specific References (6)
- Protocols
Overview
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Product nameAnti-ICAM1 antibody [EPR4776]
See all ICAM1 primary antibodies -
DescriptionRabbit monoclonal [EPR4776] to ICAM1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cytmore details
Unsuitable for: ICC -
Species reactivityReacts with: Human
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Immunogen
Recombinant fragment, corresponding to a region near the C-terminus of Human ICAM1.
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Positive control
- Lysates of the following cells: Raji, Ramos, A549 treated with TNF-alpha, A549, and HUVEC treated with TNF-alpha; Human kidney tissue and Human tonsil tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR4776
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab109361 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 89 kDa (predicted molecular weight: 58 kDa). | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| Flow Cyt | 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Application notes
Is unsuitable for ICC.
Target
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FunctionICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
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Sequence similaritiesBelongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsMonoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 3383 Human
- Omim: 147840 Human
- SwissProt: P05362 Human
- Unigene: 643447 Human
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Alternative names
- Antigen identified by monoclonal antibody BB2 antibody
- BB 2 antibody
- BB2 antibody
see all
Images
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![Flow Cytometry - Anti-ICAM1 antibody [EPR4776] (ab109361)](https://www.abcam.com/ps/products/109/ab109361/Images/ab109361-4-ab109361FC.jpg)
Flow Cytometry - Anti-ICAM1 antibody [EPR4776] (ab109361)Overlay histogram showing Ramos cells stained with ab109361 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109361, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. -
![Western blot - Anti-ICAM1 antibody [EPR4776] (ab109361)](https://www.abcam.com/ps/products/109/ab109361/Images/ICAM1-Primary-antibodies-ab109361-1.jpg)
Western blot - Anti-ICAM1 antibody [EPR4776] (ab109361)All lanes : Anti-ICAM1 antibody [EPR4776] (ab109361) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : A549 cells treated with TNF-alpha, lysate
Lane 4 : A549 cell lysate
Lane 5 : HUVEC cells treated with TNF-alpha, lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 58 kDa
Observed band size: 89 kDa (why is the actual band size different from the predicted?) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR4776] (ab109361)](https://www.abcam.com/ps/products/109/ab109361/Images/ICAM1-Primary-antibodies-ab109361-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR4776] (ab109361)ab109361, at 1/100, staining ICAM1 in Human kidney tissue by immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR4776] (ab109361)](https://www.abcam.com/ps/products/109/ab109361/Images/ICAM1-Primary-antibodies-ab109361-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR4776] (ab109361)ab1091361, at 1/100, staining ICAM1 in Human tonsil tissue by immunohistochemistry.
Protocols
References
This product has been referenced in:
- Tengroth L et al. Activation of Activin receptor-like kinases curbs mucosal inflammation and proliferation in chronic rhinosinusitis with nasal polyps. Sci Rep 8:1561 (2018). Read more (PubMed: 29367682) »
- Li HL et al. Alteration of Gut Microbiota and Inflammatory Cytokine/Chemokine Profiles in 5-Fluorouracil Induced Intestinal Mucositis. Front Cell Infect Microbiol 7:455 (2017). Read more (PubMed: 29124041) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"