Recombinant Anti-ICAM1 antibody [EPR4776] (ab109361)

Thank you for contacting us. Your credit note ID is I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Thank you for the response.

Any monoclonal antibody is produced by a single immune cell, which has been cloned. These will only recognize a single epitope of the target protein. This epitope typically is 5-10 amino acids in length. Therefore any single monoclonal antibody will only recognize a region within a single domain and not stain both the intracellular and extracellular region of the target protein.

Polyclonal antibodies are created by many different immune cells and will contain multiple epitope recognition sites. Although the amount of antibody specific to a particular epitope will vary from lot-to-lot, it is possible for a polyclonal to therefore contain antibodies which will recognize different domains.

I have reviewed the antibodies which you have listed as well as other polyclonals in our catalog to try and find a product which might be able to recognize the intracellular and extracellular regions.

ab2213Anti-ICAM1 antibody [MEM-111]: This monoclonal antibody was created with an immunogen from a whole tissue / cell preparation (Human). We have not mapped the exact location of the epitope. Therefore the epitope recognized might be intracellular, transmembrane or extracellular.

ab25375Anti-ICAM1 antibody [YN1/1.7.4]: This product is also monoclonal.The details of the immunogen for this antibody are not available. However as a monoclonal, this product will not be able to recognize sites in multiple domains.

ab7815Anti-ICAM1 antibody (Biotin): This product is a rabbit polyclonal and has been raised against the whole protein. As such it is possible that the product will contain antibodies against both extracellular and intracellular regions. We do not test for this type of response and cannot guarantee that the productwill recognizeboth domains. Please be aware that this product is also biotin labeled.

Our other rabbit polyclonal antibodies were each created from recombinant fragments and would also not be able to recognize both extracellular and intracellular domains.

I hope this information is helpful. Please do not hesitate to contact me with further questions.

While many of our ICAM1 antibodies have been tested in Western blot on human and are guaranteed to work under the terms of our Abpromise, in this instance I would recommend one of the following products. ab96485:https://www.abcam.com/ICAM1-antibody-6G12-ab96485.html Like the ab27536, this product is has been created from a recombinant protein corresponding to the extracellular portion of ICAM1. It is a mouse monoclonal ascites product which is tested in and guaranteed to work in Western blot and ELISA on human. ab20:https://www.abcam.com/ICAM1-antibody-15-2-ab20.html This product is a mouse monoclonal,Protein A purified, which has beentested inWB,Flow Cyt,IHC-Fr,IP. This product has been also been referencedin three publications. ab109361:https://www.abcam.com/ICAM1-antibody-EPR4776-ab109361.html Like ab27536 this product has been produced in rabbit. Rabbit monoclonals exhibit very high specificity and affinity. Besides being guaranteed for use in WB, this product will also work inIP,IHC-P andFlow Cyt. Each product offers the same guarantee and each would be a recommended replacement for ab27536. However each antibody does have its differences ( immunogen region, publications or rabbit affinity) which may appeal to the customer. I hope this is helpful. Please let me know if you have any questions.