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  1. Link

    icam1-antibody-yn1174-ab119871.pdf

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Immunology Cell Type Markers CD Adhesion
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Anti-ICAM1 antibody [YN1/1.7.4] (ab119871)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (49)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [YN1/1.7.4] (ab119871)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [YN1/1.7.4] (ab119871)
  • Flow Cytometry - Anti-ICAM1 antibody [YN1/1.7.4] (ab119871)

Key features and details

  • Rat monoclonal [YN1/1.7.4] to ICAM1
  • Suitable for: Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat
  • Isotype: IgG2b

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Protein
Recombinant Human ICAM1 protein (ab151393)

View more associated products

Overview

  • Product name

    Anti-ICAM1 antibody [YN1/1.7.4]
    See all ICAM1 primary antibodies
  • Description

    Rat monoclonal [YN1/1.7.4] to ICAM1
  • Host species

    Rat
  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Tissue, cells or virus corresponding to Mouse ICAM1. Mouse NS-1 cells

  • Positive control

    • This antibody gave a positive result in IHC in the following FFPE tissue: Mouse lung.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purity is > 95% (y SDS-PAGE.
  • Clonality

    Monoclonal
  • Clone number

    YN1/1.7.4
  • Isotype

    IgG2b
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • Adhesion
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Cell adhesion molecules
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • FITC Anti-ICAM1 antibody [YN1/1.7.4] (ab24869)
    • Biotin Anti-ICAM1 antibody [YN1/1.7.4] (ab25007)
    • PE Anti-ICAM1 antibody [YN1/1.7.4] (ab25553)
    • APC Anti-ICAM1 antibody [YN1/1.7.4] (ab87026)
  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    • Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rat IgG2b, kappa monoclonal [RTK4530] - Isotype control (ab18541)
  • Recombinant Protein

    • Recombinant Human ICAM1 protein (ab151393)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab119871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use a concentration of 1 µg/ml.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
Flow Cyt
Use a concentration of 1 µg/ml.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. ICAM family.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications

    Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P05362 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 15894 Mouse
    • Entrez Gene: 25464 Rat
    • SwissProt: P13597 Mouse
    • SwissProt: Q00238 Rat
    • Unigene: 435508 Mouse
    • Unigene: 12 Rat
    • Alternative names

      • Antigen identified by monoclonal antibody BB2 antibody
      • BB 2 antibody
      • BB2 antibody
      • CD 54 antibody
      • CD_antigen=CD54 antibody
      • CD54 antibody
      • Cell surface glycoprotein P3.58 antibody
      • Human rhinovirus receptor antibody
      • ICAM 1 antibody
      • ICAM-1 antibody
      • ICAM1 antibody
      • ICAM1_HUMAN antibody
      • intercellular adhesion molecule 1 (CD54) antibody
      • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
      • Intercellular adhesion molecule 1 antibody
      • Major group rhinovirus receptor antibody
      • MALA 2 antibody
      • MALA2 antibody
      • MyD 10 antibody
      • MyD10 antibody
      • P3.58 antibody
      • Surface antigen of activated B cells antibody
      • Surface antigen of activated B cells, BB2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [YN1/1.7.4] (ab119871)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [YN1/1.7.4] (ab119871)Image from Sun, Hui et al. Journal of Cellular and Molecular Medicine 19.4 (2015): 836–849. doi: 10.1111/jcmm.12521, Fig 6C. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse atheroslerotic lesions of aortic root tissue sections labeling ICAM1 with ab119871 at 1/200 dilution. The aortic root was rapidly fixed and harvested at 4°C in phosphate-buffered 4% paraformaldehyde, pH 7.0–7.4, then embedded in paraffin wax sectioned by semi-automated rotary microtome.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [YN1/1.7.4] (ab119871)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [YN1/1.7.4] (ab119871)

      IHC image of ICAM1 staining in Mouse normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with EDTA based pH 9.0 solution, (epitope retrieval solution 2) for 20 mins. The section was then incubated with ab119871, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Flow Cytometry - Anti-ICAM1 antibody [YN1/1.7.4] (ab119871)
      Flow Cytometry - Anti-ICAM1 antibody [YN1/1.7.4] (ab119871)

      Flow Cytometry analysis of mouse thymocytes labeling ICAM1 with Anti-ICAM1 antibody [YN1/1.7.4] (ab119871).

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (49)

    Publishing research using ab119871? Please let us know so that we can cite the reference in this datasheet.

    ab119871 has been referenced in 49 publications.

    • Tan Y  et al. SERCA Overexpression Improves Mitochondrial Quality Control and Attenuates Cardiac Microvascular Ischemia-Reperfusion Injury. Mol Ther Nucleic Acids 22:696-707 (2020). PubMed: 33230467
    • Zhou L  et al. DHZCP Modulates Microglial M1/M2 Polarization via the p38 and TLR4/NF-?B Signaling Pathways in LPS-Stimulated Microglial Cells. Front Pharmacol 11:1126 (2020). PubMed: 32848745
    • Zhou H  et al. DNA-PKcs promotes cardiac ischemia reperfusion injury through mitigating BI-1-governed mitochondrial homeostasis. Basic Res Cardiol 115:11 (2020). PubMed: 31919590
    • Lu J  et al. Urate-lowering therapy alleviates atherosclerosis inflammatory response factors and neointimal lesions in a mouse model of induced carotid atherosclerosis. FEBS J 286:1346-1359 (2019). PubMed: 30690853
    • Bontemps A  et al. In Vivo Comparison of the Phenotypic Aspects and Molecular Mechanisms of Two Nephrotoxic Agents, Sodium Fluoride and Uranyl Nitrate. Int J Environ Res Public Health 16:N/A (2019). PubMed: 30934888
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-ICAM1 antibody [YN1/1.7.4]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Antigen retrieval step
    None
    Sample
    Mouse Tissue sections (Olfactory epithelium)
    Specification
    Olfactory epithelium
    Permeabilization
    Yes - .1% TritonX-100 in block
    Fixative
    1% Paraformaldehyde+lysine+periodate (1%PLP)
    Read More

    Dr. Schwob Lab

    Verified customer

    Submitted Jun 10 2014

    Question

    We ordered the following product (ORDER #1154284 ) and have questions about the product. Do you have information of the correct molecular weight of the protein recognized by this antibody (ab119871)?

    https://www.abcam.com/ICAM1-antibody-YN1-1-7-4-ab119871.html

    I am looking forward to hearing from you soon. Thanks a lot!

    Read More

    Abcam community

    Verified customer

    Asked on Oct 23 2012

    Answer

    Thank you for contacting us.

    The predicted size, based on the amino acid sequence before any post-translation modifications, is 58 kDa. I do not have the western blot data for this particular antibody, but 58 kDa is the size of the proteins recognized by another ICAM1 antibody, ab20:

    https://www.abcam.com/ICAM1-antibody-152-ab20.html .

    On the other hand, other antibodies recognize bands at higher molecular weights, which may represent glycosylated ICAM1, as seen for example in the blot on the datasheet for ab109361:

    https://www.abcam.com/ICAM1-antibody-EPR4776-ab109361.html

    If you are having difficulty understanding your results with ab119871, please contact us and describe your results and protocol.

    Read More

    Abcam Scientific Support

    Answered on Oct 23 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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