Anti-ICAM1 antibody [YN1/1.7.4] (ab119871)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse atheroslerotic lesions of aortic root tissue sections labeling ICAM1 with ab119871 at 1/200 dilution. The aortic root was rapidly fixed and harvested at 4°C in phosphate-buffered 4% paraformaldehyde, pH 7.0–7.4, then embedded in paraffin wax sectioned by semi-automated rotary microtome.

ab119871 staining ICAM1 in mouse olfactory epithelium tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with 1% paraformaldehyde + lysine + periodate (1% PLP) and permeablized and blocked with 10% donkey serum + 5% nonfat dry milk + 4% BSA + 1% triton X-100. The sample was incubated with primary antibody (1/200) for 1 hour at 27°C. An Alexa Fluor^® 488-conjugated donkey anti-rat polyclonal (1/150) was used as the secondary antibody.

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IHC image of ICAM1 staining in Mouse normal lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with EDTA based pH 9.0 solution, (epitope retrieval solution 2) for 20 mins. The section was then incubated with ab119871, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Flow Cytometry analysis of mouse thymocytes labeling ICAM1 with Anti-ICAM1 antibody [YN1/1.7.4] (ab119871).