Anti-ICAM1 antibody [YN1/1.7.4] (ab119871)
Key features and details
- Rat monoclonal [YN1/1.7.4] to ICAM1
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG2b
Overview
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Product name
Anti-ICAM1 antibody [YN1/1.7.4]
See all ICAM1 primary antibodies -
Description
Rat monoclonal [YN1/1.7.4] to ICAM1 -
Host species
Rat -
Tested applications
Suitable for: Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Tissue, cells or virus corresponding to Mouse ICAM1. Mouse NS-1 cells
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Positive control
- This antibody gave a positive result in IHC in the following FFPE tissue: Mouse lung.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purity is > 95% (y SDS-PAGE. -
Clonality
Monoclonal -
Clone number
YN1/1.7.4 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119871 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use a concentration of 1 µg/ml.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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Flow Cyt
Use a concentration of 1 µg/ml. ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. -
Sequence similarities
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsMonoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 15894 Mouse
- Entrez Gene: 25464 Rat
- SwissProt: P13597 Mouse
- SwissProt: Q00238 Rat
- Unigene: 435508 Mouse
- Unigene: 12 Rat
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Alternative names
- Antigen identified by monoclonal antibody BB2 antibody
- BB 2 antibody
- BB2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [YN1/1.7.4] (ab119871)Image from Sun, Hui et al. Journal of Cellular and Molecular Medicine 19.4 (2015): 836–849. doi: 10.1111/jcmm.12521, Fig 6C. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse atheroslerotic lesions of aortic root tissue sections labeling ICAM1 with ab119871 at 1/200 dilution. The aortic root was rapidly fixed and harvested at 4°C in phosphate-buffered 4% paraformaldehyde, pH 7.0–7.4, then embedded in paraffin wax sectioned by semi-automated rotary microtome.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [YN1/1.7.4] (ab119871)
IHC image of ICAM1 staining in Mouse normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with EDTA based pH 9.0 solution, (epitope retrieval solution 2) for 20 mins. The section was then incubated with ab119871, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Flow Cytometry analysis of mouse thymocytes labeling ICAM1 with Anti-ICAM1 antibody [YN1/1.7.4] (ab119871).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (49)
ab119871 has been referenced in 49 publications.
- Tan Y et al. SERCA Overexpression Improves Mitochondrial Quality Control and Attenuates Cardiac Microvascular Ischemia-Reperfusion Injury. Mol Ther Nucleic Acids 22:696-707 (2020). PubMed: 33230467
- Zhou L et al. DHZCP Modulates Microglial M1/M2 Polarization via the p38 and TLR4/NF-?B Signaling Pathways in LPS-Stimulated Microglial Cells. Front Pharmacol 11:1126 (2020). PubMed: 32848745
- Zhou H et al. DNA-PKcs promotes cardiac ischemia reperfusion injury through mitigating BI-1-governed mitochondrial homeostasis. Basic Res Cardiol 115:11 (2020). PubMed: 31919590
- Lu J et al. Urate-lowering therapy alleviates atherosclerosis inflammatory response factors and neointimal lesions in a mouse model of induced carotid atherosclerosis. FEBS J 286:1346-1359 (2019). PubMed: 30690853
- Bontemps A et al. In Vivo Comparison of the Phenotypic Aspects and Molecular Mechanisms of Two Nephrotoxic Agents, Sodium Fluoride and Uranyl Nitrate. Int J Environ Res Public Health 16:N/A (2019). PubMed: 30934888