• Product name
    Anti-ICAM1 antibody [YN1/1.7.4]
    See all ICAM1 primary antibodies
  • Description
    Rat monoclonal [YN1/1.7.4] to ICAM1
  • Host species
  • Tested applications
    Suitable for: IP, IHC-Fr, Blocking, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control
    • PMA or LPS stimulated T-cell line such as EL-4 or 18-81



Our Abpromise guarantee covers the use of ab25375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. PubMed: 2573511
IHC-Fr Use at an assay dependent concentration. PubMed: 8943409
Blocking Use at an assay dependent concentration. PubMed: 2981916
WB Use at an assay dependent concentration. Detects a band of approximately 95 kDa (predicted molecular weight: 85-110 kDa). PubMed: 8095510
Flow Cyt Use at an assay dependent concentration.


  • Function
    ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. ICAM family.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen identified by monoclonal antibody BB2 antibody
    • BB 2 antibody
    • BB2 antibody
    • CD 54 antibody
    • CD_antigen=CD54 antibody
    • CD54 antibody
    • Cell surface glycoprotein P3.58 antibody
    • Human rhinovirus receptor antibody
    • ICAM 1 antibody
    • ICAM-1 antibody
    • ICAM1 antibody
    • ICAM1_HUMAN antibody
    • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
    • Intercellular adhesion molecule 1 antibody
    • Major group rhinovirus receptor antibody
    • MALA 2 antibody
    • MALA2 antibody
    • MyD 10 antibody
    • MyD10 antibody
    • P3.58 antibody
    • Surface antigen of activated B cells, BB2 antibody
    see all


  • Flow Cytometry analysis of BALB/c mouse splenocytes labeling ICAM1 with ab25375 at 0.3 μg/106 cells (purple). A Mouse Anti-Rat IgG2b-CY5 was used as the secondary antibody. Grey - Isotype Control, Rat IgG2b-UNLB, followed by Mouse Anti-Rat IgG2b-CY5.


This product has been referenced in:
  • De Niz M  et al. Plasmodium gametocytes display homing and vascular transmigration in the host bone marrow. Sci Adv 4:eaat3775 (2018). Read more (PubMed: 29806032) »
  • Xu Y  et al. Regulation of endothelial intracellular adenosine via adenosine kinase epigenetically modulates vascular inflammation. Nat Commun 8:943 (2017). Read more (PubMed: 29038540) »

See all 18 Publications for this product

Customer reviews and Q&As

Thank you for your call today and for your interest in these antibodies.

Since we do not have a CD54 antibody that hasalready been tested in IHC-P with mouse samples, I can offer a discount off a future purchase if you'd liketest a CD54 antib...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for the response.

Any monoclonal antibody is produced by a single immune cell, which has been cloned. These will only recognize a single epitope of the target protein. This epitope typically is 5-10 amino acids in length. Therefore...

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Perdona por la tardanza en contestar, estaba chequeando con mis compañeros del laboratorio las distintas opciones de reactividad cruzada de este anticuerpo con conejo. Desgraciadamente no hay muchos datos al respecto, ya que ninguno de ellos se ...

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Thank you for your enquiry. I have enquired at the originator of the product but unfortunately, they do not have the information that you are requesting. I am sorry I can't be of more assistance in this occasion. If there is anything else I can...

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Thank you for providing these details regarding your protocol. Ab25375 should detect a band at approximately 58 kDa; did you see a band at this size? This particular antibody has only so far been tested for cross-reactivity with mouse and has not yet b...

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