Recombinant
RabMAb

Recombinant Anti-ICAM2 antibody [EPR18231-183] - BSA and Azide free (ab224531)

Overview

  • Product name

    Anti-ICAM2 antibody [EPR18231-183] - BSA and Azide free
    See all ICAM2 primary antibodies
  • Description

    Rabbit monoclonal [EPR18231-183] to ICAM2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment aa 1-250. The exact sequence is proprietary.
    Database link: P35330

  • Positive control

    • IHC: Mouse spleen tissue.
  • General notes

    Ab224531 is the carrier-free version of ab189463. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab224531 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab224531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 31 kDa.
IP Use at an assay dependent concentration.

Target

  • Relevance

    ICAM2 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin like C2 type domains, and bind to the leukocyte adhesion LFA1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA1 dependent cell adhesion. It mediates adhesive interactions important for antigen specific immune response, NK cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.
  • Cellular localization

    Membrane; Single-pass type I membrane protein
  • Database links

  • Alternative names

    • CD102 antibody
    • CD102 antigen antibody
    • ICAM 2 antibody
    • Intercellular adhesion molecule 2 antibody
    • Ly60 antibody
    see all

Images

  • ICAM2 was immunoprecipitated from 0.35 mg of bEnd.3 (mouse brain endothelioma cell line) whole cell lysate with ab189463 at 1/60 dilution. Western blot was performed from the immunoprecipitate using ab189463 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: bEnd.3 whole cell lysate 10 µg (Input). 

    Lane 2: ab189463 IP in bEnd.3 whole cell lysate (+). 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab189463 in bEnd.3 whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 30 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189463).

  • Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling ICAM2 with ab189463 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Positive staining on vascular endothelium of mouse cerebral cortex (PMID: 12516546). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189463).

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling ICAM2 with ab189463 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Positive staining on vascular endothelium of mouse spleen (PMID: 12516546). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189463).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab224531 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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