Recombinant
RabMAb

Recombinant Anti-ICAM2 antibody [EPR19114-113] (ab197570)

Overview

  • Product name

    Anti-ICAM2 antibody [EPR19114-113]
    See all ICAM2 primary antibodies
  • Description

    Rabbit monoclonal [EPR19114-113] to ICAM2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human ICAM2 aa 1-250. The exact sequence is proprietary.
    Database link: P13598

  • Positive control

    • WB: Jurkat cell lysate; human fetal kidney and fetal spleen lysate. IHC-P: Human lung and spleen tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab197570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 31 kDa).
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Relevance

    ICAM2 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin like C2 type domains, and bind to the leukocyte adhesion LFA1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA1 dependent cell adhesion. It mediates adhesive interactions important for antigen specific immune response, NK cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.
  • Cellular localization

    Membrane; Single-pass type I membrane protein
  • Database links

  • Alternative names

    • CD102 antibody
    • CD102 antigen antibody
    • ICAM 2 antibody
    • Intercellular adhesion molecule 2 antibody
    • Ly60 antibody
    see all

Images

  • Anti-ICAM2 antibody [EPR19114-113] (ab197570) at 1/5000 dilution + Jurkat (human T cell leukemia T lymphocyte) lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 31 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 1676048).

  • Immunohistochemical analysis of paraffin-embedded human lung tissue labeling ICAM2 with ab197570 at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on vascular endothelium of human lung (PMID: 1676048) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • All lanes : Anti-ICAM2 antibody [EPR19114-113] (ab197570) at 1/100 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

    Developed using the ECL technique.

    Predicted band size: 31 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?


    Exposure time: 20 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling ICAM2 with ab197570 at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on vascular endothelium of human spleen (PMID: 1676048) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

References

ab197570 has not yet been referenced specifically in any publications.

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