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    icam3-antibody-epr3994-123-ab109405.pdf

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Signal Transduction Protein Phosphorylation pSer / pThr
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RecombinantRabMAb

Recombinant Anti-ICAM3 antibody [EPR3994-123] (ab109405)

  • Datasheet
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Western blot - Anti-ICAM3 antibody [EPR3994-123] (ab109405)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)
  • Immunocytochemistry - Anti-ICAM3 antibody [EPR3994-123] (ab109405)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)
  • Anti-ICAM3 antibody [EPR3994-123] (ab109405)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3994-123] to ICAM3
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-ICAM3 antibody [EPR3994-123]
    See all ICAM3 primary antibodies
  • Description

    Rabbit monoclonal [EPR3994-123] to ICAM3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • U937, HeLa, Jurkat, HuT-78, Ramos, and THP-1 cell lysates; Human spleen, colon and tonsil tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3994-123
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • pSer / pThr
    • Signal Transduction
    • Adapters
    • Transmembrane
    • Immunology
    • Cell Type Markers
    • CD
    • Adhesion

Associated products

  • Alternative Versions

    • Anti-ICAM3 antibody [EPR3994-123] - BSA and Azide free (ab247851)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat membrane extract lysate (ab30130)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
1/100 - 1/250.
WB
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
ICC
1/100 - 1/250.
WB
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. ICAM family.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications

    Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues.
    N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P32942 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3385 Human
    • Omim: 146631 Human
    • SwissProt: P32942 Human
    • Unigene: 654563 Human
    • Alternative names

      • CD50 antibody
      • CDW 50 antibody
      • CDw50 antibody
      • ICAM 3 antibody
      • ICAM-3 antibody
      • ICAM-R antibody
      • ICAM3 antibody
      • ICAM3_HUMAN antibody
      • ICAMR antibody
      • Intercellular adhesion molecule 3 antibody
      see all

    Images

    • Western blot - Anti-ICAM3 antibody [EPR3994-123] (ab109405)
      Western blot - Anti-ICAM3 antibody [EPR3994-123] (ab109405)
      All lanes : Anti-ICAM3 antibody [EPR3994-123] (ab109405) at 1/1000 dilution

      Lane 1 : U937 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HuT-78 cell lysate
      Lane 4 : Ramos cell lysate
      Lane 5 : THP-1 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 60 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)

      Immunohistochemical analysis of ICAM3 in paraffin-embedded Human spleen tissue using ab109405 at 1/500 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry - Anti-ICAM3 antibody [EPR3994-123] (ab109405)
      Immunocytochemistry - Anti-ICAM3 antibody [EPR3994-123] (ab109405)
      This data was developed using the same antibody clone in a different buffer formulation (ab247851).
      Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) labeling ICAM3 with purified ab247851 at 1/500 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.24 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
      Negative control: PBS instead of the primary antibody.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)

      Immunohistochemical analysis of ICAM3 in paraffin-embedded Human colon tissue using ab109405 at 1/500 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)

      Immunohistochemical analysis of ICAM3 in paraffin-embedded Human tonsil tissue using ab109405 at 1/500dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-ICAM3 antibody [EPR3994-123] (ab109405)
      Anti-ICAM3 antibody [EPR3994-123] (ab109405)

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (1)

    Publishing research using ab109405? Please let us know so that we can cite the reference in this datasheet.

    ab109405 has been referenced in 1 publication.

    • Shen W  et al. ICAM3 mediates tumor metastasis via a LFA-1-ICAM3-ERM dependent manner. Biochim Biophys Acta 1864:2566-2578 (2018). WB . PubMed: 29729315

    Customer reviews and Q&As

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