Recombinant Anti-ICAM3 antibody [EPR3994-123] (ab109405)
![Western blot - Anti-ICAM3 antibody [EPR3994-123] (ab109405)](https://www.abcam.com/ps/products/109/ab109405/Images/ab109405-122218-anti-icam3-antibody-epr3994-123-western-blot.jpg "Western blot - Anti-ICAM3 antibody [EPR3994-123] (ab109405)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3994-123] to ICAM3
- Suitable for: ICC, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ICAM3 antibody [EPR3994-123]
See all ICAM3 primary antibodies -
Description
Rabbit monoclonal [EPR3994-123] to ICAM3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- U937, HeLa, Jurkat, HuT-78, Ramos, and THP-1 cell lysates; Human spleen, colon and tonsil tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3994-123 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
1/100 - 1/250.
|
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
|
|
| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
ICC
1/100 - 1/250. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 60 kDa. |
|
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2. -
Sequence similarities
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsUpon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues.
N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3385 Human
- Omim: 146631 Human
- SwissProt: P32942 Human
- Unigene: 654563 Human
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Alternative names
- CD50 antibody
- CDW 50 antibody
- CDw50 antibody
see all
Images
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Western blot - Anti-ICAM3 antibody [EPR3994-123] (ab109405)All lanes : Anti-ICAM3 antibody [EPR3994-123] (ab109405) at 1/1000 dilution
Lane 1 : U937 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HuT-78 cell lysate
Lane 4 : Ramos cell lysate
Lane 5 : THP-1 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 60 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)](https://www.abcam.com/ps/products/109/ab109405/Images/ab109405-122217-anti-icam3-antibody-epr3994-123-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)Immunohistochemical analysis of ICAM3 in paraffin-embedded Human spleen tissue using ab109405 at 1/500 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry - Anti-ICAM3 antibody [EPR3994-123] (ab109405)](https://www.abcam.com/ps/products/109/ab109405/Images/ab109405-5-anti-icam3-antibody-epr3994-123-immunocytochemistry-hela-human.jpg)
Immunocytochemistry - Anti-ICAM3 antibody [EPR3994-123] (ab109405)This data was developed using the same antibody clone in a different buffer formulation (ab247851).
Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) labeling ICAM3 with purified ab247851 at 1/500 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.24 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)](https://www.abcam.com/ps/products/109/ab109405/Images/ab109405-122216-anti-icam3-antibody-epr3994-123-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)Immunohistochemical analysis of ICAM3 in paraffin-embedded Human colon tissue using ab109405 at 1/500 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)](https://www.abcam.com/ps/products/109/ab109405/Images/ab109405-122215-anti-icam3-antibody-epr3994-123-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)Immunohistochemical analysis of ICAM3 in paraffin-embedded Human tonsil tissue using ab109405 at 1/500dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-ICAM3 antibody [EPR3994-123] (ab109405)](https://www.abcam.com/ps/products/109/ab109405/Images/ab109405-6-benefits-of-recombinant-antibodies.png)
Anti-ICAM3 antibody [EPR3994-123] (ab109405)
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab109405 has been referenced in 1 publication.
- Shen W et al. ICAM3 mediates tumor metastasis via a LFA-1-ICAM3-ERM dependent manner. Biochim Biophys Acta 1864:2566-2578 (2018). WB . PubMed: 29729315
