Recombinant
RabMAb

Recombinant Anti-ICAM3 antibody [EPR3994-123] (ab109405)

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Overview

  • Product name

    Anti-ICAM3 antibody [EPR3994-123]
    See all ICAM3 primary antibodies

  • Description

    Rabbit monoclonal [EPR3994-123] to ICAM3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IP, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human ICAM3 (extracellular). The exact sequence is proprietary.

  • Positive control

    • U937, Jurkat, HuT-78, Ramos, and THP-1 cell lysates; Human spleen, colon and tonsil tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IP 1/10 - 1/100.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2.

  • Sequence similarities

    Belongs to the immunoglobulin superfamily. ICAM family.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

  • Post-translational
    modifications

    Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues.
    N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD50 antibody
      • CDW 50 antibody
      • CDw50 antibody
      • ICAM 3 antibody
      • ICAM-3 antibody
      • ICAM-R antibody
      • ICAM3 antibody
      • ICAM3_HUMAN antibody
      • ICAMR antibody
      • Intercellular adhesion molecule 3 antibody
      see all

    Images

    • Western blot - Anti-ICAM3 antibody [EPR3994-123] (ab109405)
      Western blot - Anti-ICAM3 antibody [EPR3994-123] (ab109405)
      All lanes : Anti-ICAM3 antibody [EPR3994-123] (ab109405) at 1/1000 dilution

      Lane 1 : U937 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HuT-78 cell lysate
      Lane 4 : Ramos cell lysate
      Lane 5 : THP-1 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 60 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)

      Immunohistochemical analysis of ICAM3 in paraffin-embedded Human spleen tissue using ab109405 at 1/500 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)

      Immunohistochemical analysis of ICAM3 in paraffin-embedded Human colon tissue using ab109405 at 1/500 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (ab109405)

      Immunohistochemical analysis of ICAM3 in paraffin-embedded Human tonsil tissue using ab109405 at 1/500dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Shen W  et al. ICAM3 mediates tumor metastasis via a LFA-1-ICAM3-ERM dependent manner. Biochim Biophys Acta 1864:2566-2578 (2018). WB . Read more (PubMed: 29729315) »
    See 1 Publication for this product

    Publishing research using ab109405? Please let us know so that we can cite the reference in this datasheet.

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